Autoantibodies to 60 kDa SS-A/Ro yield a specific nuclear myriad discrete fine speckled immunofluorescence pattern

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dc.contributor.authorDellavance, Alessandra
dc.contributor.authorAlvarenga, Rossana Rassi [UNIFESP]
dc.contributor.authorRodrigues, Silvia Helena [UNIFESP]
dc.contributor.authorBarbosa, Silvia Helena
dc.contributor.authorPinto Camilo, Amandia Cristina
dc.contributor.authorOkamoto Shiguedomi, Herika Santiago
dc.contributor.authorRodrigues, Silvia Sanchez
dc.contributor.authorSilva, Cristiane Gallindo
dc.contributor.authorCoelho Andrade, Luis Eduardo [UNIFESP]
dc.contributor.institutionFleury Med & Hlth Labs
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:31:31Z
dc.date.available2016-01-24T14:31:31Z
dc.date.issued2013-04-01
dc.description.abstractObjectives: To report on a novel immunofluorescence pattern specifically associated with antibodies to SS-A/Ro.Methods: A novel immunofluorescence pattern, herein designated SS-A/Ro pattern, was characterized as myriad discrete fine speckles throughout the nucleus. Eighty-six sequential samples presenting the SS-A/Ro pattern and 64 samples presenting non-SS-A/Ro nuclear fine speckled pattern at the ANA-HEp-2 routine were screened for SS-A/Ro reactivity. Conversely, 48 samples with known reactivity to 60 kDa SS-A/Ro, 13 samples with exclusive reactivity to 52 kDa SS-A/Ro, and 48 SS-A/Ro-negative samples were analyzed for the ANA-HEp-2 pattern.Results: Eighty-five of the 86 samples (98.8%) presenting the SS-A/Ro pattern and 15 of the 64 (23.4%) samples with non-SS-A/Ro fine speckled pattern reacted with 60 kDa SS-A/Ro. Conversely, 44 (91.6%) of 48 samples with known reactivity to 60 kDa SS-A/Ro presented the SS-A/Ro pattern and four (8.4%) presented non-SS-A/Ro fine speckled pattern. None of the 48 anti-SS-A/Ro-negative samples and none of anti-52 kDa SS-A/Ro-positive samples yielded the SS-A/Ro pattern. This immunofluorescence pattern was observed in different commercial HEp-2 cell slides and in homemade HEp-2 cell slides.Conclusions: the SS-A/Ro pattern belongs to the group of immunofluorescence patterns that hold strong association with the respective autoantibody specificities, such as those associated with CENP-F and NuMA-1. the identification of the SS-A/Ro pattern at the ANA-HEp-2 screening routine shall lead to specific tests for the identification of anti-SS-A/Ro antibodies. (C) 2013 Elsevier B.V. All rights reserved.en
dc.description.affiliationFleury Med & Hlth Labs, Div Res & Dev, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Div Rheumatol, BR-04023900 São Paulo, Brazil
dc.description.affiliationFleury Med & Hlth Labs, Div Immunol, São Paulo, Brazil
dc.description.affiliationFleury Med & Hlth Labs, Reagents Prod Div, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Div Rheumatol, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent35-40
dc.identifierhttp://dx.doi.org/10.1016/j.jim.2013.01.006
dc.identifier.citationJournal of Immunological Methods. Amsterdam: Elsevier B.V., v. 390, n. 1-2, p. 35-40, 2013.
dc.identifier.doi10.1016/j.jim.2013.01.006
dc.identifier.issn0022-1759
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36161
dc.identifier.wosWOS:000317380500005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Immunological Methods
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectAntinuclear antibodiesen
dc.subjectAutoantibodiesen
dc.subjectSS-A/Roen
dc.subjectSystemic lupus erythematosusen
dc.subjectSjogren's syndromeen
dc.titleAutoantibodies to 60 kDa SS-A/Ro yield a specific nuclear myriad discrete fine speckled immunofluorescence patternen
dc.typeinfo:eu-repo/semantics/article
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