Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells

Página do item simplificado
dc.citation.issue1
dc.citation.volumev. 7
dc.contributor.authorRibeiro, Kleber Silva [UNIFESP]
dc.contributor.authorVasconcellos, Camilla Ioshida [UNIFESP]
dc.contributor.authorSoares, Rodrigo Pedro
dc.contributor.authorMendes, Maria Tays
dc.contributor.authorEllis, Cameron C.
dc.contributor.authorAguilera-Flores, Marcela
dc.contributor.authorAlmeida, Igor Correia de
dc.contributor.authorSchenkman, Sergio [UNIFESP]
dc.contributor.authorIwai, Leo Kei
dc.contributor.authorTorrecilhas, Ana Claudia [UNIFESP]
dc.coverageAbingdon
dc.date.accessioned2020-07-20T16:31:00Z
dc.date.available2020-07-20T16:31:00Z
dc.date.issued2018
dc.description.abstractTrypanosoma cruzi, the aetiologic agent of Chagas disease, releases vesicles containing a wide range of surface molecules known to affect the host immunological responses and the cellular infectivity. Here, we compared the secretome of two distinct strains (Y and YuYu) of T. cruzi, which were previously shown to differentially modulate host innate and acquired immune responses. Tissue culture-derived trypomastigotes of both strains secreted extracellular vesicles (EVs), as demonstrated by electron scanning microscopy. EVs were purified by exclusion chromatography or ultracentrifugation and quantitated using nanoparticle tracking analysis. Trypomastigotes from YuYu strain released higher number of EVs than those from Y strain, enriched with virulence factors trans-sialidase (TS) and cruzipain. Proteomic analysis confirmed the increased abundance of proteins coded by the TS gene family, mucin-like glycoproteins, and some typical exosomal proteins in the YuYu strain, which also showed considerable differences between purified EVs and vesicle-free fraction as compared to the Y strain. To evaluate whether such differences were related to parasite infectivity, J774 macrophages and LLC-MK2 kidney cells were preincubated with purified EVs from both strains and then infected with Y strain trypomastigotes. EVs released by YuYu strain caused a lower infection but higher intracellular proliferation in J774 macrophages than EVs from Y strain. In contrast, YuYu strain-derived EVs caused higher infection of LLC-MK2 cells than Y strain-derived EVs. In conclusion, quantitative and qualitative differences in EVs and secreted proteins from different T. cruzi strains may correlate with infectivity/virulence during the host-parasite interaction.en
dc.description.affiliationUNIFESP, Dept Ciencias Farmaceut, Rua Sao Nicolau 210, BR-09913030 Diadema Sao Paulo, Brazil
dc.description.affiliationFIOCRUZ MG, Inst Rene Rachou, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Texas El Paso, Dept Biol Sci, Border Biomed Res Ctr, El Paso, TX 79968 USA
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationCtr Toxins Immune Response & Cell Signaling CeTIC, LETA, Inst Butantan, Sao Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Dept Ciencias Farmaceut, Rua Sao Nicolau 210, BR-09913030 Diadema Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipCoordination of Superior Level Staff Improvement of Higher Education Personnel (CAPES)
dc.description.sponsorshipCNPq
dc.description.sponsorshipFAPEMIG
dc.description.sponsorshipScience Without Borders Program
dc.description.sponsorshipNational Institute of General Medical Sciences (NIGMS)
dc.description.sponsorshipIDFAPESP: 2016-01917-3
dc.description.sponsorshipIDFAPESP: 2013/07467-1
dc.description.sponsorshipIDCNPq: 305065/2016-5
dc.description.sponsorshipIDFAPEMIG: PPM-00102-16
dc.description.sponsorshipIDNIGMS: 2G12MD007592
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1080/20013078.2018.1463779
dc.identifier.citationJournal Of Extracellular Vesicles. Abingdon, v. 7, n. 1, p. -, 2018.
dc.identifier.doi10.1080/20013078.2018.1463779
dc.identifier.fileWOS000430234200001.pdf
dc.identifier.issn2001-3078
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55633
dc.identifier.wosWOS:000430234200001
dc.language.isoeng
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofJournal Of Extracellular Vesicles
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectExtracellular vesiclesen
dc.subjectT. cruzi host interactionen
dc.titleProteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cellsen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000430234200001.pdf
Tamanho:
2.74 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
ICAQF - Artigos
EPM - Artigos