Plasmatic higher levels of homocysteine in Non-alcoholic fatty liver disease (NAFLD)

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dc.contributor.authorCarvalho, Sylene Coutinho Rampche de
dc.contributor.authorMuniz, Maria Tereza Cartaxo
dc.contributor.authorSiqueira, Maria Deozete Vieira
dc.contributor.authorSiqueira, Erika Rabelo Forte
dc.contributor.authorGomes, Adriana Vieira
dc.contributor.authorSilva, Karina Alves
dc.contributor.authorBezerra, Lais Carvalho Luma
dc.contributor.authorD'Almeida, Vania [UNIFESP]
dc.contributor.authorOliveira, Claudia Pinto Marques Souza de
dc.contributor.authorPereira, Leila Maria M. Beltrao
dc.contributor.institutionUniv Pernambuco
dc.contributor.institutionLiver Inst Pernambuco
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInst Figado Pernambuco
dc.date.accessioned2016-01-24T14:31:33Z
dc.date.available2016-01-24T14:31:33Z
dc.date.issued2013-04-02
dc.description.abstractBackground: Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease, which includes a spectrum of hepatic pathology such as simple steatosis, steatohepatitis, fibrosis and cirrhosis. the increased serum levels of homocysteine (Hcy) may be associated with hepatic fat accumulation. Genetic mutations in the folate route may only mildly impair Hcy metabolism. the aim of this study was to investigate the relation between liver steatosis with plasma homocysteine level and MTHFR C677T and A1298C polymorphisms in Brazilian patients with NAFLD.Methods: Thirty-five patients diagnosed with NAFLD by liver biopsy and forty-five healthy controls neither age nor sex matched were genotyped for C677T and A1298C MTHFR polymorphisms using PCR-RFLP and PCR-ASA, respectively, and Hcy was determined by HPLC. All patients were negative for markers of Wilson's, hemochromatosis and autoimmune diseases. Their daily alcohol intake was less than 100 g/week. A set of metabolic and serum lipid markers were also measured at the time of liver biopsies.Results: the plasma Hcy level was higher in NAFLD patients compared to the control group (p = 0.0341). No statistical difference for genotypes 677C/T (p = 0.110) and 1298A/C (p = 0.343) in patients with NAFLD and control subjects was observed. the genotypes distribution was in Hardy-Weinberg equilibrium (677C/T p = 0.694 and 1298 A/C p = 0.188). the group of patients and controls showed a statistically significant difference (p < 0.001) for BMI and HOMA_IR, similarly to HDL cholesterol levels (p < 0,006), AST, ALT, gamma GT, AP and triglycerides levels (p < 0.001). A negative correlation was observed between levels of vitamin B12 and Hcy concentration (p = 0.005).Conclusion: Our results indicate that plasma Hcy was higher in NAFLD than controls. the MTHFR C677T and A1298C polymorphisms did not differ significantly between groups, despite the 677TT homozygous frequency was higher in patients (17.14%) than in controls (677TT = 4.44%) (p > 0.05). the suggested genetic susceptibility to the MTHFR C677T and A1298C should be confirmed in large population based studies.en
dc.description.affiliationUniv Pernambuco, Sch Med, Recife, PE, Brazil
dc.description.affiliationUniv Pernambuco, Pediat Hematol & Oncol Ctr, Recife, PE, Brazil
dc.description.affiliationLiver Inst Pernambuco, Recife, PE, Brazil
dc.description.affiliationUniv Pernambuco, Inst Biol Sci, Recife, PE, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pediat, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, São Paulo, Brazil
dc.description.affiliationInst Figado Pernambuco, BR-50100130 Santo Amaro Recife, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pediat, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent5
dc.identifierhttp://dx.doi.org/10.1186/1475-2891-12-37
dc.identifier.citationNutrition Journal. London: Biomed Central Ltd, v. 12, 5 p., 2013.
dc.identifier.doi10.1186/1475-2891-12-37
dc.identifier.fileWOS000317666000001.pdf
dc.identifier.issn1475-2891
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36192
dc.identifier.wosWOS:000317666000001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofNutrition Journal
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFatty liveren
dc.subjectNon-alcoholic steatohepatitisen
dc.subjectMethylenetetrahydrofolate reductase (MTHFR)en
dc.subjectOxidative stressen
dc.subjectPolymorphismsen
dc.titlePlasmatic higher levels of homocysteine in Non-alcoholic fatty liver disease (NAFLD)en
dc.typeinfo:eu-repo/semantics/article
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