Cellulolytic and proteolytic ability of bacteria isolated from gastrointestinal tract and composting of a hippopotamus

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dc.citation.volume6
dc.contributor.authorda Cruz Ramos, Geomarcia Feitosa [UNIFESP]
dc.contributor.authorRamos, Patricia Locosque
dc.contributor.authorZambrano Passarini, Michel Rodrigo [UNIFESP]
dc.contributor.authorVieira Silveira, Marghuel A. [UNIFESP]
dc.contributor.authorOkamoto, Debora Noma [UNIFESP]
dc.contributor.authorGoncalves de Oliveira, Lilian Caroline [UNIFESP]
dc.contributor.authorZezzo, Larissa Vieira [UNIFESP]
dc.contributor.authorMarem, Alyne [UNIFESP]
dc.contributor.authorSantos Rocha, Rafael Costa [UNIFESP]
dc.contributor.authorda Cruz, Joao Batista
dc.contributor.authorJuliano, Luiz [UNIFESP]
dc.contributor.authorde Vasconcellos, Suzan Pantaroto [UNIFESP]
dc.coverageLondon
dc.date.accessioned2020-08-21T17:00:12Z
dc.date.available2020-08-21T17:00:12Z
dc.date.issued2016
dc.description.abstractThe bioprospection for cellulase and protease producers is a promise strategy for the discovery of potential biocatalysts for use in hydrolysis of lignocellulosic materials as well as proteic residues. These enzymes can increment and turn viable the production of second generation ethanol from different and alternative sources. In this context, the goal of this study was the investigation of cellulolytic and proteolytic abilities of bacteria isolated from the gastrointestinal tract of a hippopotamus as well as from its composting process. It is important to highlight that hippopotamus gastrointestinal samples were a non-typical sources of efficient hydrolytic bacteria with potential for application in biotechnological industries, like biofuel production. Looking for this, a total of 159 bacteria were isolated, which were submitted to qualitative and quantitative enzymatic assays. Proteolytic analyzes were conducted through the evaluation of fluorescent probes. Qualitative assays for cellulolytic abilities revealed 70 positive hits. After quantitative analyzes, 44 % of these positive hits were selected, but five (5) strains showed cellulolytic activity up to 11,8 FPU/mL. Regarding to proteolytic activities, six (6) strains showed activity above 10 %, which overpassed results described in the literature. Molecular analyzes based on the identification of 16S rDNA, revealed that all the selected bacterial isolates were affiliated to Bacillus genus. In summary, these results strongly indicate that the isolated bacteria from a hippopotamus can be a potential source of interesting biocatalysts with cellulolytic and proteolytic activities, with relevance for industrial applications.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Biol Sci, Rua Sao Nicolau 210, BR-09913030 Sao Paulo, Brazil
dc.description.affiliationSao Paulo Zoo Pk Fdn, Av Miguel Estefano 4241, BR-04301905 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biophys, Rua 3 Maio,100, BR-04044020 Sao Paulo, SP, Brazil
dc.description.affiliationFed Univ Latin Amer Integrat, Latin Amer Inst Life Sci & Nat, Av Tarquinio Joslin Santos 1000, BR-85870901 Foz Do Iguacu, Parana, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biol Sci, Rua Sao Nicolau 210, BR-09913030 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biophys, Rua 3 Maio,100, BR-04044020 Sao Paulo, SP, Brazil
dc.description.provenanceMade available in DSpace on 2020-08-21T17:00:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2016. Added 1 bitstream(s) on 2020-08-27T14:20:57Z : No. of bitstreams: 1 WOS000371385600001.pdf: 1387149 bytes, checksum: 29a62371d23223fab7271fa7e702bca2 (MD5)en
dc.description.sourceWeb of Science
dc.description.sponsorshipBrazilian research agency (FAPESP)
dc.description.sponsorshipBrazilian research agency (CAPES)
dc.description.sponsorshipBrazilian research agency (CNPq)
dc.description.sponsorshipIDFAPESP: 2010/51992-5
dc.description.sponsorshipIDCNPq: 475166/2013-2
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1186/s13568-016-0188-x
dc.identifier.citationAmb Express. London, v. 6, p. -, 2016.
dc.identifier.doi10.1186/s13568-016-0188-x
dc.identifier.fileWOS000371385600001.pdf
dc.identifier.issn2191-0855
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57905
dc.identifier.wosWOS:000371385600001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofAmb Express
dc.rightsAcesso aberto
dc.subjectProteaseen
dc.subjectCellulaseen
dc.subjectScreeningen
dc.subjectBiofuelen
dc.subjectBacillusen
dc.titleCellulolytic and proteolytic ability of bacteria isolated from gastrointestinal tract and composting of a hippopotamusen
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