Aerobic exercise training rescues protein quality control disruption on white skeletal muscle induced by chronic kidney disease in rats

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dc.citation.issue3
dc.citation.volume22
dc.contributor.authorMoraes, Wilson Max Almeida Monteiro de [UNIFESP]
dc.contributor.authorSouza, Pamella Ramona Moraes de
dc.contributor.authorPaixao, Nathalie Alves de
dc.contributor.authorSousa, Luis Gustavo Oliveira de
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorMarshall, Andrea G.
dc.contributor.authorPrestes, Jonato
dc.contributor.authorIrigoyen, Maria Claudia
dc.contributor.authorBrum, Patricia Chakur
dc.contributor.authorMedeiros, Alessandra [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.coverageHoboken
dc.date.accessioned2020-07-08T13:09:32Z
dc.date.available2020-07-08T13:09:32Z
dc.date.issued2018
dc.description.abstractWe tested whether aerobic exercise training (AET) would modulate the skeletal muscle protein quality control (PQC) in a model of chronic kidney disease (CKD) in rats. Adult Wistar rats were evaluated in four groups: control (CS) or trained (CE), and 5/6 nephrectomy sedentary (5/6NxS) or trained (5/6NxE). Exercised rats were submitted to treadmill exercise (60 min., five times/wk for 2 months). We evaluated motor performance (tolerance to exercise on the treadmill and rotarod), cross-sectional area (CSA), gene and protein levels related to the unfolded protein response (UPR), protein synthesis/survive and apoptosis signalling, accumulated misfolded proteins, chymotrypsin-like proteasome activity (UPS activity), redox balance and heat-shock protein (HSP) levels in the tibialis anterior. 5/6NxS presented a trend towards to atrophy, with a reduction in motor performance, down-regulation of protein synthesis and up-regulation of apoptosis signalling; increases in UPS activity, misfolded proteins, GRP78, derlin, HSP27 and HSP70 protein levels, ATF4 and GRP78 genes; and increase in oxidative damage compared to CS group. In 5/6NxE, we observed a restoration in exercise tolerance, accumulated misfolded proteins, UPS activity, protein synthesis/apoptosis signalling, derlin, HSPs protein levels as well as increase in ATF4, GRP78 genes and ATF6α protein levels accompanied by a decrease in oxidative damage and increased catalase and glutathione peroxidase activities. The results suggest a disruption of PQC in white muscle fibres of CKD rats previous to the atrophy. AET can rescue this disruption for the UPR, prevent accumulated misfolded proteins and reduce oxidative damage, HSPs protein levels and exercise tolerance.en
dc.description.affiliationUniv Fed Sao Paulo, Biosci Dept, Santos, Brazil
dc.description.affiliationCatholic Univ Brasilia UCB, Postgrad Program Phys Educ, Brasilia, DF, Brazil
dc.description.affiliationNove de Julho Univ UNINOVE, Dept Postgrad Med, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Sch Phys Educ & Sport, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, Sao Paulo, Brazil
dc.description.affiliationUniv Calif Davis, Dept Neurobiol Physiol & Behav, Davis, CA 95616 USA
dc.description.affiliationUniv Sao Paulo, Sch Med, Inst Heart, Hypertens Unit, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Biosci Dept, Santos, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDCAPES: 12/51090-1
dc.description.sponsorshipIDFAPESP: 15/198936-0
dc.format.extent1452-1463
dc.identifierhttps://dx.doi.org/10.1111/jcmm.13374
dc.identifier.citationJournal Of Cellular And Molecular Medicine. Hoboken, v. 22, n. 3, p. 1452-1463, 2018.
dc.identifier.doi10.1111/jcmm.13374
dc.identifier.fileWOS000426069300007.pdf
dc.identifier.issn1582-4934
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54064
dc.identifier.wosWOS:000426069300007
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofJournal Of Cellular And Molecular Medicine
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChronic kidney diseaseen
dc.subjectAerobic exercise trainingen
dc.subjectSkeletal muscleen
dc.subjectProtein quality controlen
dc.subjectUnfolded protein responseen
dc.titleAerobic exercise training rescues protein quality control disruption on white skeletal muscle induced by chronic kidney disease in ratsen
dc.typeinfo:eu-repo/semantics/article
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