Production, characterization, and application of antibodies against heat-labile type-I toxin for detection of enterotoxigenic Escherichia coli

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dc.contributor.authorMenezes, Caroline A
dc.contributor.authorImamura, Sergio Y
dc.contributor.authorTrabulsi, Luiz Rachid
dc.contributor.authorFernandes-Filho, Antônio
dc.contributor.authorMartinez, Marina B
dc.contributor.authorGuth, Beatriz Ernestina Cabilio [UNIFESP]
dc.contributor.authorGirão, Dennys M
dc.contributor.authorPiazza, Roxane Mf
dc.contributor.institutionInstituto Butantan Laboratório de Bacteriologia
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro Instituto Prof. Paulo de Góes Departamento de Microbiologia Médica
dc.date.accessioned2015-06-14T13:36:35Z
dc.date.available2015-06-14T13:36:35Z
dc.date.issued2006-12-01
dc.description.abstractStrains of enterotoxigenic Escherichia coli (ETEC) are responsible for significant rates of morbidity and mortality among children, particularly in developing countries. The majority of clinical and public health laboratories are capable of isolating and identifying Salmonella, Shigella, Campylobacter, and Escherichia coli O157:H7 from stool samples, but ETEC cannot be identified by routine methods. The method most often used to identify ETEC is polymerase chain reaction for heat-stable and heat-labile enterotoxin genes, and subsequent serotyping, but most clinical and public health laboratories do not have the capacity or resources to perform these tests. In this study, polyclonal rabbit and monoclonal mouse IgG2b antibodies against ETEC heat-labile toxin-I (LT) were characterized and the potential applicability of a capture assay was analyzed. IgG-enriched fractions from rabbit polyclonal and the IgG2b monoclonal antibodies recognized LT in a conformational shape and they were excellent tools for detection of LT-producing strains. These findings indicate that the capture immunoassay could be used as a diagnostic assay of ETEC LT-producing strains in routine diagnosis and in epidemiological studies of diarrhea in developing countries as enzyme linked immunosorbent assay techniques remain as effective and economical choice for the detection of specific pathogen antigens in cultures.en
dc.description.affiliationInstituto Butantan Laboratório de Bacteriologia
dc.description.affiliationUniversidade de São Paulo Faculdade de Ciências Farmacêuticas Departamento de Análises Clínicas e Toxicológicas
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departamento de Microbiologia, Imunologia e Parasitologia
dc.description.affiliationUniversidade Federal do Rio de Janeiro Instituto Prof. Paulo de Góes Departamento de Microbiologia Médica
dc.description.affiliationUnifespUNIFESP, Depto. de Microbiologia, Imunologia e Parasitologia
dc.description.sourceSciELO
dc.format.extent875-880
dc.identifierhttp://dx.doi.org/10.1590/S0074-02762006000800009
dc.identifier.citationMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 101, n. 8, p. 875-880, 2006.
dc.identifier.doi10.1590/S0074-02762006000800009
dc.identifier.fileS0074-02762006000800009.pdf
dc.identifier.issn0074-0276
dc.identifier.scieloS0074-02762006000800009
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/3402
dc.identifier.wosWOS:000243649600009
dc.language.isoeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectdetectionen
dc.subjectantibodiesen
dc.subjectheat-labile toxinen
dc.subjectEscherichia colien
dc.titleProduction, characterization, and application of antibodies against heat-labile type-I toxin for detection of enterotoxigenic Escherichia colien
dc.typeinfo:eu-repo/semantics/article
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