Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer

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dc.contributor.authorLima, Eleonidas Moura
dc.contributor.authorLeal, Mariana Ferreira [UNIFESP]
dc.contributor.authorBurbano, Rommel Rodríguez [UNIFESP]
dc.contributor.authorKhayat, Andre Salim
dc.contributor.authorAssumpção, Paulo Pimentes de [UNIFESP]
dc.contributor.authorBello, Maria Jose
dc.contributor.authorRey, J.a.
dc.contributor.authorSmith, Marilia de Arruda Cardoso [UNIFESP]
dc.contributor.authorCasartelli, Carla
dc.contributor.institutionUniversidade Federal do Piauí Colegiado de Biomedicina
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal do Pará Instituto de Ciências Biológicas Laboratório de Citogenética Humana
dc.contributor.institutionHospital João de Barros Barreto Serviço de Cirurgia
dc.contributor.institutionInstituto de Investigaciones Biomedicas
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-06-14T13:38:35Z
dc.date.available2015-06-14T13:38:35Z
dc.date.issued2008-06-01
dc.description.abstractGastric cancer is the forth most frequent malignancy and the second most common cause of cancer death worldwide. DNA methylation is the most studied epigenetic alteration, occurring through a methyl radical addition to the cytosine base adjacent to guanine. Many tumor genes are inactivated by DNA methylation in gastric cancer. We evaluated the DNA methylation status of ANAPC1, CDKN2A and TP53 by methylation-specific PCR in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosa in individuals from Northern Brazil. All gastric cancer samples were advanced stage adenocarcinomas. Gastric samples were surgically obtained at the João de Barros Barreto University Hospital, State of Pará, and were stored at -80°C before DNA extraction. Patients had never been submitted to chemotherapy or radiotherapy, nor did they have any other diagnosed cancer. None of the gastric cancer samples presented methylated DNA sequences for ANAPC1 and TP53. CDKN2A methylation was not detected in any normal gastric mucosa; however, the CDKN2A promoter was methylated in 30.4% of gastric cancer samples, with 35% methylation in diffuse-type and 26.9% in intestinal-type cancers. CDKN2A methylation was associated with the carcinogenesis process for ~30% diffuse-type and intestinal-type compared to non-neoplastic samples. Thus, ANAPC1 and TP53 methylation was probably not implicated in gastric carcinogenesis in our samples. CDKN2A can be implicated in the carcinogenesis process of only a subset of gastric neoplasias.en
dc.description.affiliationUniversidade Federal do Piauí Colegiado de Biomedicina
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Morfologia
dc.description.affiliationUniversidade Federal do Pará Instituto de Ciências Biológicas Laboratório de Citogenética Humana
dc.description.affiliationHospital João de Barros Barreto Serviço de Cirurgia
dc.description.affiliationInstituto de Investigaciones Biomedicas
dc.description.affiliationUniversidade de São Paulo Faculdade de Medicina de Ribeirão Preto Departamento de Genética
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Morfologia
dc.description.sourceSciELO
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFINEP/CT-INFRA
dc.description.sponsorshipFAEPA
dc.description.sponsorshipIDFINEP/CT-INFRA: 0927-03
dc.format.extent539-543
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2008000600017
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 41, n. 6, p. 539-543, 2008.
dc.identifier.doi10.1590/S0100-879X2008000600017
dc.identifier.fileS0100-879X2008000600017.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2008000600017
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/4408
dc.identifier.wosWOS:000258766600017
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDNA methylationen
dc.subjectGastric canceren
dc.subjectANAPC1en
dc.subjectCDKN2Aen
dc.subjectTP53en
dc.titleMethylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric canceren
dc.typeinfo:eu-repo/semantics/article
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