Evaluation of photodynamic therapy in adhesion protein expression

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dc.contributor.authorPacheco-Soares, Cristina
dc.contributor.authorMaftou-Costa, Maira [UNIFESP]
dc.contributor.authorDa Cunha Menezes Costa, Carolina Genuncio
dc.contributor.authorDe Siqueira Silva, Andreza Cristina
dc.contributor.authorMoraes, Karen C. M.
dc.contributor.institutionUniv Vale do Paraiba
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:37:38Z
dc.date.available2016-01-24T14:37:38Z
dc.date.issued2014-08-01
dc.description.abstractPhotodynamic therapy (PDT) is a treatment modality that has clinical applications in both non-neoplastic and neoplastic diseases. PDT involves a light-sensitive compound (photosensitizer), light and molecular oxygen. This procedure may lead to several different cellular responses, including cell death. Alterations in the attachment of cancer cells to the substratum and to each other are important consequences of photodynamic treatment. PDT may lead to changes in the expression of cellular adhesion structure and cytoskeleton integrity, which are key factors in decreasing tumor metastatic potential. HEp-2 cells were photosensitized with aluminum phthalocyanine tetrasulfonate and zinc phthalocyanine, and the proteins beta 1-integrin and focal adhesion kinase (FAK) were assayed using fluorescence microscopy. the verification of expression changes in the genes for FAK and beta 1 integrin were performed by reverse transcription-polymerase chain reaction (RT-PCR). the results revealed that HEp-2 cells do not express beta-integrin or FAK 12 h following PDT. It was concluded that the PDT reduces the adhesive ability of HEp-2 cells, inhibiting their metastatic potential. the present study aimed to analyze the changes in the expression and organization of cellular adhesion elements and the subsequent metastatic potential of HEp-2 cells following PDT treatment.en
dc.description.affiliationUniv Vale do Paraiba, Inst Res & Dev, Lab Dynam Cellular Compartments, BR-12244000 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pharmacol, BR-04021001 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Biosci Rio Claro, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pharmacol, BR-04021001 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2006/06736-5
dc.format.extent714-718
dc.identifierhttp://dx.doi.org/10.3892/ol.2014.2149
dc.identifier.citationOncology Letters. Athens: Spandidos Publ Ltd, v. 8, n. 2, p. 714-718, 2014.
dc.identifier.doi10.3892/ol.2014.2149
dc.identifier.issn1792-1074
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38021
dc.identifier.wosWOS:000339127300041
dc.language.isoeng
dc.publisherSpandidos Publ Ltd
dc.relation.ispartofOncology Letters
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectphthalocyaninesen
dc.subjectfocal adhesionen
dc.subjectbeta 1-integrinen
dc.subjectreverse transcription-polymerase chain reactionen
dc.subjectcell cultureen
dc.titleEvaluation of photodynamic therapy in adhesion protein expressionen
dc.typeinfo:eu-repo/semantics/article
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