Immunophenotypic characterization of peripheral T lymphocytes in Mycobacterium tuberculosis infection and disease

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dc.contributor.authorRodrigues, Denise do Socorro da Silva [UNIFESP]
dc.contributor.authorMedeiros, Eduardo Alexandrino Servolo de [UNIFESP]
dc.contributor.authorWeckx, Lily Yin [UNIFESP]
dc.contributor.authorBonnez, W.
dc.contributor.authorSalomão, Reinaldo [UNIFESP]
dc.contributor.authorKallas, Esper Georges [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Rochester
dc.date.accessioned2016-01-24T12:33:19Z
dc.date.available2016-01-24T12:33:19Z
dc.date.issued2002-04-01
dc.description.abstractThe cellular immune response probably plays a pivotal role in determining the clinical outcome after exposure to Mycobacterium tuberculosis. We used multi-parameter flow-cytometry to evaluate the distribution of T-lymphocyte subsets during infection and disease caused by M. tuberculosis. Samples were obtained from 71 volunteers to identify the T CD4(+) and CD8(+) lymphocyte numbers, and the activation plus memory/naive phenotypes, as defined by CD38, HLA-DR, CD45RA and CD27 markers. Subjects were divided into 18 healthy volunteers without detectable reaction to purified protein derivative (PPD-), 18 health care workers with a recent conversion to PPD, 20 patients with active pulmonary tuberculosis (TBC) and 15 patients with treated TBC at 6 months of therapy. By multiple-comparison analyses, the T CD4(+) lymphocyte number of the TBC group was lower than the PPD- group (P < 0.05). Ibis difference was apparently lost after treatment. the higher and the lower number of naive T CD4(+) cells was observed in the PPD- and TB C group, respectively. CD8(+) T lymphocytes were also statistically different among the four groups (P = 0.0002), lower in the TBC group (P < 0.05). CD8(+) T lymphocyte activation was evaluated by the CD38 and HLA-DR surface expression. the percentage distribution of these markers was statistically different between the four groups (P = 0.0055). TBC patients had a higher percentage of CD38(+) cells and mean fluorescence index, suggesting an overall increase of cell activation. These results suggest that peripheral T lymphocytes reflect cellular activation during TBC, along with possible redistribution of naive, memory/effector and late differentiated memory/effector phenotypes in the peripheral blood after infection and disease caused by M. tuberculosis.en
dc.description.affiliationUniversidade Federal de São Paulo, LKab Imunol, Disciplina Doencas Infecc & Parasitarias, Escola Paulista Med,Infect Dis Discipline, BR-04039032 São Paulo, Brazil
dc.description.affiliationUniv Rochester, Infect Dis Unit, Rochester, NY 14627 USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, LKab Imunol, Disciplina Doencas Infecc & Parasitarias, Escola Paulista Med,Infect Dis Discipline, BR-04039032 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent149-154
dc.identifierhttp://dx.doi.org/10.1046/j.1365-2249.2002.01809.x
dc.identifier.citationClinical and Experimental Immunology. Oxford: Blackwell Publishing Ltd, v. 128, n. 1, p. 149-154, 2002.
dc.identifier.doi10.1046/j.1365-2249.2002.01809.x
dc.identifier.issn0009-9104
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26805
dc.identifier.wosWOS:000177079300021
dc.language.isoeng
dc.publisherBlackwell Publishing Ltd
dc.relation.ispartofClinical and Experimental Immunology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMycobacterium tuberculosisen
dc.subjecttuberculosisen
dc.subjectlymphocyteen
dc.subjectimmunophenotypingen
dc.titleImmunophenotypic characterization of peripheral T lymphocytes in Mycobacterium tuberculosis infection and diseaseen
dc.typeinfo:eu-repo/semantics/article
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