Evidence of genotypic diversity among Candida auris isolates by multilocus sequence typing, matrix-assisted laser desorption ionization time-of-flight mass spectrometry and amplified fragment length polymorphism

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dc.citation.issue3
dc.citation.volume22
dc.contributor.authorPrakash, A.
dc.contributor.authorSharma, C.
dc.contributor.authorSingh, A.
dc.contributor.authorSingh, P. Kumar
dc.contributor.authorKumar, A.
dc.contributor.authorHagen, F.
dc.contributor.authorGovender, N. P.
dc.contributor.authorColombo, A. L. [UNIFESP]
dc.contributor.authorMeis, J. F.
dc.contributor.authorChowdhary, A.
dc.coverageOxford
dc.date.accessioned2020-08-21T17:00:08Z
dc.date.available2020-08-21T17:00:08Z
dc.date.issued2016
dc.description.abstractCandida auris is a multidrug-resistant nosocomial bloodstream pathogen that has been reported from Asian countries and South Africa. Herein, we studied the population structure and genetic relatedness among 104 global C. auris isolates from India, South Africa and Brazil using multilocus sequence typing (MLST), amplified fragment length polymorphism (AFLP) fingerprinting and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). RPB1, RPB2 and internal transcribed spacer (ITS) and D1/D2 regions of the ribosomal DNA were sequenced for MLST. Further, genetic variation and proteomic assessment was carried out using AFLP and MALDI-TOF MS, respectively. Both MLST and AFLP typing clearly demarcated two major clusters comprising Indian and Brazilian isolates. However, the South African isolates were randomly distributed, suggesting different genotypes. MALDI-TOF MS spectral profiling also revealed evidence of geographical clustering but did not correlate fully with the genotyping methods. Notably, overall the population structure of C. auris showed evidence of geographical clustering by all the three techniques analysed. Antifungal susceptibility testing by the CLSI microbroth dilution method revealed that fluconazole had limited activity against 87% of isolates (MIC90, 64 mg/L). Also, MIC90 of AMB was 4 mg/L. Candida auris is emerging as an important yeast pathogen globally and requires reproducible laboratory methods for identification and typing. Evaluation of MALDI-TOF MS as a typing method for this yeast is warranted. Clinical Microbiology and Infection (C) 2015 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Delhi, Vallabhbhai Patel Chest Inst, Dept Med Mycol, Delhi 110007, India
dc.description.affiliationAmrita Inst Med Sci, Dept Microbiol, Kochi, India
dc.description.affiliationCanisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, Nijmegen, Netherlands
dc.description.affiliationTrop & Hosp Infect, Ctr Opportunist, Natl Inst Communicable Dis, Johannesburg, South Africa
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Disciplina Infectol, Lab Especial Micol, Sao Paulo, SP, Brazil
dc.description.affiliationRadboudumc, Dept Med Microbiol, Nijmegen, Netherlands
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Disciplina Infectol, Lab Especial Micol, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipUniversity Grants Commission Research Fellowship, India
dc.description.sponsorshipIndian Council of Medical Research, Government of India, New Delhi, India
dc.description.sponsorshipIDUniversity Grants Commission Research Fellowship: F.2-15/2003 SA-I
dc.description.sponsorshipIDIndian Council of Medical Research, Government of India: 80/815/2013-ECD-I
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1016/j.cmi.2015.10.022
dc.identifier.citationClinical Microbiology And Infection. Oxford, v. 22, n. 3, p. -, 2016.
dc.identifier.doi10.1016/j.cmi.2015.10.022
dc.identifier.issn1198-743X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57883
dc.identifier.wosWOS:000372083500018
dc.language.isoeng
dc.publisherElsevier Sci Ltd
dc.relation.ispartofClinical Microbiology And Infection
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAmplified fragment length polymorphismen
dc.subjectCandida aurisen
dc.subjectD1/D2en
dc.subjectinternal transcribed spaceren
dc.subjectmatrix-assisted laser desorption ionization time-of-flight mass spectrometryen
dc.subjectRPB1en
dc.subjectRPB2en
dc.titleEvidence of genotypic diversity among Candida auris isolates by multilocus sequence typing, matrix-assisted laser desorption ionization time-of-flight mass spectrometry and amplified fragment length polymorphismen
dc.typeinfo:eu-repo/semantics/article
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