Effects of Lopap on human endothelial cells and platelets

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dc.contributor.authorChudzinski-Tavassi, Ana Marisa [UNIFESP]
dc.contributor.authorSchattner, M.
dc.contributor.authorFritzen, Marcio [UNIFESP]
dc.contributor.authorPozner, R. G.
dc.contributor.authorReis, C. V.
dc.contributor.authorLourenco, Dayse Maria [UNIFESP]
dc.contributor.authorLazzari, M. A.
dc.contributor.institutionFAPESP
dc.contributor.institutionNatl Acad Med
dc.contributor.institutionConsejo Nacl Invest Cient & Tecn
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T17:30:23Z
dc.date.available2018-06-15T17:30:23Z
dc.date.issued2001-05-01
dc.description.abstractSevere consumption coagulopathy has been detected in rats after Lopap (a prothrombin activator from Lonomia obliqua caterpillar bristles) infusion and in humans after accidental contact with L. obliqua bristles. However, platelet count and antithrombin (AT) levels were only modestly affected, suggesting that a different form of blood coagulation activation may be involved in this hemorrhagic syndrome. Here we describe that Lopap had no effect on aggregation of washed human platelets induced by several agonists, suggesting that it might not impair platelet function in vivo. AT was able to inhibit the amidolytic activity of thrombin generated by incubation of Lopap with prothrombin in a purified system, which maybe different from that generated by the prothrombinase complex in vivo. The surface expression of both ICAM-1 and E-selectin but not of VCAM-1 was upregulated by Lopap in cultured HUVEC, suggesting that it may behave differently from other mediators, such as thrombin and tumor necrosis factor-alpha. Copyright (C) 2002 S. Karger AG, Basel.en
dc.description.affiliationFAPESP, Biochem & Biophys Lab, Butantan Inst, CEPID,Ctr Appl Toxinol, BR-05503900 Sao Paulo, Brazil
dc.description.affiliationNatl Acad Med, Hematol Res Inst, Buenos Aires, DF, Argentina
dc.description.affiliationConsejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina
dc.description.affiliationUNIFESP, Dept Hematol, Sao Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Dept Hematol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent257-265
dc.identifierhttps://doi.org/10.1159/000048071
dc.identifier.citationHaemostasis. Basel: Karger, v. 31, n. 3-6, p. 257-265, 2001.
dc.identifier.doi10.1159/000048071
dc.identifier.issn0301-0147
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43739
dc.identifier.wosWOS:000174839200019
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofHaemostasis
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.karger.com/Services/RightsPermissions
dc.subjectLopapen
dc.subjectprothrombin activatoren
dc.subjectLonomia obliquaen
dc.subjectplateletsen
dc.subjectICAM-1en
dc.subjectE-selectinen
dc.subjectendothelial cellsen
dc.titleEffects of Lopap on human endothelial cells and plateletsen
dc.typeinfo:eu-repo/semantics/article
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