Biological and immunological characterization of recombinant Yellow Fever 17D Viruses expressing a Trypanosoma cruzi Amastigote Surface Protein-2 CD8(+) T cell epitope at two distinct regions of the genome

Página do item simplificado
dc.contributor.authorNogueira, Raquel T.
dc.contributor.authorNogueira, Alanderson R.
dc.contributor.authorPereira, Mirian C. S.
dc.contributor.authorRodrigues, Mauricio Martins [UNIFESP]
dc.contributor.authorGaller, Ricardo
dc.contributor.authorBonaldo, Myrna C.
dc.contributor.institutionFundacao Oswaldo Cruz
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:06:18Z
dc.date.available2016-01-24T14:06:18Z
dc.date.issued2011-03-18
dc.description.abstractBackground: the attenuated Yellow fever (YF) 17D vaccine virus is one of the safest and most effective viral vaccines administered to humans, in which it elicits a polyvalent immune response. Herein, we used the YF 17D backbone to express a Trypanosoma cruzi CD8(+) T cell epitope from the Amastigote Surface Protein 2 (ASP-2) to provide further evidence for the potential of this virus to express foreign epitopes. the TEWETGQI CD8(+) T cell epitope was cloned and expressed based on two different genomic insertion sites: in the fg loop of the viral Envelope protein and the protease cleavage site between the NS2B and NS3. We investigated whether the site of expression had any influence on immunogenicity of this model epitope.Results: Recombinant viruses replicated similarly to vaccine virus YF 17D in cell culture and remained genetically stable after several serial passages in Vero cells. Immunogenicity studies revealed that both recombinant viruses elicited neutralizing antibodies to the YF virus as well as generated an antigen-specific gamma interferon mediated T-cell response in immunized mice. the recombinant viruses displayed a more attenuated phenotype than the YF 17DD vaccine counterpart in mice. Vaccination of a mouse lineage highly susceptible to infection by T. cruzi with a homologous prime-boost regimen of recombinant YF viruses elicited TEWETGQI specific CD8(+) T cells which might be correlated with a delay in mouse mortality after a challenge with a lethal dose of T. cruzi.Conclusions: We conclude that the YF 17D platform is useful to express T. cruzi (Protozoan) antigens at different functional regions of its genome with minimal reduction of vector fitness. in addition, the model T. cruzi epitope expressed at different regions of the YF 17D genome elicited a similar T cell-based immune response, suggesting that both expression sites are useful. However, the epitope as such is not protective and it remains to be seen whether expression of larger domains of ASP-2, which include the TEWETGQI epitope, will elicit better T-CD8+ responses to the latter. It is likely that additional antigens and recombinant virus formulations will be necessary to generate a protective response.en
dc.description.affiliationFundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Biol Mol Flavivirus, BR-21045900 Rio de Janeiro, Brazil
dc.description.affiliationFundacao Oswaldo Cruz, Inst Tecnol Imunobiol, BR-21045900 Rio de Janeiro, Brazil
dc.description.affiliationFundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Ultraestrutura Celular, BR-21045900 Rio de Janeiro, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Ctr Terapia Celular Mol CTCMol, BR-04044010 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Ctr Terapia Celular Mol CTCMol, BR-04044010 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipMillennium Institute for Vaccine Development and Technology
dc.description.sponsorshipMillennium Institute for Gene Therapy (Brazil)
dc.description.sponsorshipIDMillennium Institute for Vaccine Development and Technology: CNPq - 420067/2005-1
dc.format.extent13
dc.identifierhttp://dx.doi.org/10.1186/1743-422X-8-127
dc.identifier.citationVirology Journal. London: Biomed Central Ltd, v. 8, 13 p., 2011.
dc.identifier.doi10.1186/1743-422X-8-127
dc.identifier.fileWOS000288912600001.pdf
dc.identifier.issn1743-422X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33560
dc.identifier.wosWOS:000288912600001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofVirology Journal
dc.rightsAcesso aberto
dc.titleBiological and immunological characterization of recombinant Yellow Fever 17D Viruses expressing a Trypanosoma cruzi Amastigote Surface Protein-2 CD8(+) T cell epitope at two distinct regions of the genomeen
dc.typeArtigo
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000288912600001.pdf
Tamanho:
2.17 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
EPM - Artigos