High Risk HPV E6/E7 Oncoprotein Expression in Women with High Grade Squamous Intraepithelial Lesion

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dc.citation.issue3
dc.citation.volume38
dc.contributor.authorCordeiro Valenca, Jefferson Elias
dc.contributor.authorGoncalves, Ana Katherine
dc.contributor.authorCotrim Guerreiro da Silva, Ismael Dale [UNIFESP]
dc.contributor.authorEleuterio Junior, Jose
dc.contributor.authorda Silva, Terezinha Tenerio
dc.contributor.authorBruneska, Danyelly
dc.contributor.authorde Alencar Ximenes, Ricardo Arraes
dc.coverageRio De Janeiro Rj
dc.date.accessioned2020-08-21T17:00:20Z
dc.date.available2020-08-21T17:00:20Z
dc.date.issued2016
dc.description.abstractPurpose To correlate the expression of high-risk HPV E6 mRNA with pap smear, colposcopy, and biopsy results in women with high grade squamous intraepithelial lesion (HSIL). Methods A cross-sectional study was performed on women referred for primary care services after cytological diagnosis of HSIL. We evaluated the expression of E6/E7 mRNA of HPV types 16,18,31,33, and 45 and correlated the results with those of Pap smear, colposcopy, and biopsy. For amplification/detection of mRNA E6/E7 we used NucliSENSEasyQ kit to detect HPV mRNA by polymerase chain reaction with primers/probes for HPV types 16, 18, 31, 33, and 45. Results Out of 128 valid tests, the results of 30 (23.4%) tests were negative and 98 (70%) tests were positive. Only one type of HPV was detected in 87.7% of the E6/E7 mRNA positive cases. HPV16 was detected in 61.2% of the cases, followed by HPV33 (26.5%), HPV31 (17.3%), HPV18 (10%), and HPV45 (4.08%). Pap smear tests revealed that the E6/E7 test was positive in 107 (83.8%) women with atypical squamous cells high grade (ASC-H), HSIL, or higher. The E6/E7 test was positive in 69 (57.5%) specimens presenting negative cytology results. When analyzing the association with colposcopy results, the frequency of positive E6/E7 results increased with the severity of the injury, ranging from 57.1% in women without colposcopy-detected injury to 86.5% in those with higher levels of colposcopy findings. Of the 111 women who underwent biopsy and E6/E7 testing, the E6/E7 test was positive in 84.7% of the women who presented with lesions of cervical intraepithelial neoplasia (CIN) grade 2 or higher. Finally, 41.2% of women with a negative biopsy presented a positive E6/E7 test. Conclusions E6/E7 mRNA expression was higher in women with HSIL and CIN grade 2 or higher.en
dc.description.affiliationUniv Fed Pernambuco UFPE, Gynecol & Obstet Dept, Recife, PE, Brazil
dc.description.affiliationUniv Fed Rio Grande do Norte, Gynecol & Obstet Dept, Natal, RN, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Gynecol & Obstet Dept, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Ceara, Gynecol & Obstet Dept, Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Fed Pernambuco, Biochem Dept, Recife, PE, Brazil
dc.description.affiliationUniv Fed Pernambuco, Trop Med Dept, Recife, PE, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Gynecol & Obstet Dept, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent154-159
dc.identifierhttp://dx.doi.org/10.1055/s-0036-1580713
dc.identifier.citationRevista Brasileira De Ginecologia E Obstetricia. Rio De Janeiro Rj, v. 38, n. 3, p. 154-159, 2016.
dc.identifier.doi10.1055/s-0036-1580713
dc.identifier.fileS0100-72032016000300154-en.pdf
dc.identifier.issn0100-7203
dc.identifier.scieloS0100-72032016000300154
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57943
dc.identifier.wosWOS:000410243300007
dc.language.isoeng
dc.publisherFederacao Brasileira Soc Ginecologia & Obstetricia-Febrasgo
dc.relation.ispartofRevista Brasileira De Ginecologia E Obstetricia
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcolposcopyen
dc.subjectHPVen
dc.subjectmRNAen
dc.subjectPap smearen
dc.subjectwomenen
dc.titleHigh Risk HPV E6/E7 Oncoprotein Expression in Women with High Grade Squamous Intraepithelial Lesionen
dc.typeinfo:eu-repo/semantics/article
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