alpha-Tocopherol supplementation avoids apoptosis in the anal sphincter

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dc.contributor.authorSilva, Romualdo Correa da [UNIFESP]
dc.contributor.authorReis, Leonardo Oliveira
dc.contributor.authorLorenzetti, Fabio [UNIFESP]
dc.contributor.authorPalma, Paulo
dc.contributor.authorOrtiz, Valdemar [UNIFESP]
dc.contributor.authorDambros, Miriam [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2016-01-24T14:26:57Z
dc.date.available2016-01-24T14:26:57Z
dc.date.issued2012-03-01
dc.description.abstractPurpose: To analyze the influence of alpha-tocopherol supplementation on the levels of oxidative stress and apoptosis rates in the anal sphincter induced by orchiectomy in rats. Methods: Forty male Wistar rats weighing 250-300 g, were divided into four groups and sacrificed 8 weeks after: I- Control: sham; II- Orchiectomy: bilateral orchiectomy; III- Pre Orchiectomy Tocopherol: alpha-tocopherol supplementation for 4 weeks preceding bilateral orchiectomy; IV- Orchiectomy Full Tocopherol: alpha-tocopherol supplementation for 4 weeks before and 8 weeks after bilateral orchiectomy. the anal sphincter was analyzed stereologically to evaluate the density of collagen and the muscle fibers. the oxidative stress and the apoptosis were determined with 8-isprostane and caspase-3, respectively. Results: the collagen fibers concentration was statistically greater in Orchiectomy group than the others. the muscle fibers concentration was higher in Control and Orchiectomy Full Tocopherol than Orchiectomy and Pre Orchiectomy Tocopherol groups. Orchiectomy group showed higher 8-isoprostane concentrations compared to the other groups (p < 0.0003). Pre Orchiectomy Tocopherol and Orchiectomy Full Tocopherol groups presented caspase-3 levels lower than the Orchiectomy group (0.0072). Conclusion: Vitamin supplementation with alpha-tocopherol for 12 weeks had the highest protection against bilateral orchiectomy generation of reactive oxygen species as well as apoptosis in the muscle fibers of the anal sphincter of rats.en
dc.description.affiliationUniversidade Federal de São Paulo, Div Geriatr Urol, Escola Paulista Med, São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Div Urol, UNICAMP, Campinas, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Geriatr Urol, Escola Paulista Med, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent48-53
dc.identifierhttp://dx.doi.org/10.3109/13685538.2011.593657
dc.identifier.citationAging Male. London: Informa Healthcare, v. 15, n. 1, p. 48-53, 2012.
dc.identifier.doi10.3109/13685538.2011.593657
dc.identifier.issn1368-5538
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34701
dc.identifier.wosWOS:000299840800008
dc.language.isoeng
dc.publisherInforma Healthcare
dc.relation.ispartofAging Male
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.subjectapoptosisen
dc.subjectanal sphincteren
dc.subjectalpha-tocopherolen
dc.subjectoxidative stressen
dc.subjectantioxidantsen
dc.subjectvitaminsen
dc.titlealpha-Tocopherol supplementation avoids apoptosis in the anal sphincteren
dc.typeinfo:eu-repo/semantics/article
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