Low-level laser therapy (LLLT) acts as cAMP-elevating agent in acute respiratory distress syndrome

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dc.contributor.authorLima, Flavia Mafra de
dc.contributor.authorMoreira, Leonardo M.
dc.contributor.authorVillaverde, A. B.
dc.contributor.authorAlbertini, Regiane
dc.contributor.authorCastro-Faria-Neto, Hugo C.
dc.contributor.authorAimbire, Flavio [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionRes & Dev Inst IP&D
dc.contributor.institutionDept Biosyst Engn
dc.contributor.institutionUnicastelo
dc.contributor.institutionCtr Univ Nove Julho Uninove
dc.contributor.institutionFiocruz MS
dc.date.accessioned2016-01-24T14:16:41Z
dc.date.available2016-01-24T14:16:41Z
dc.date.issued2011-05-01
dc.description.abstractThe aim of this work was to investigate if the low-level laser therapy (LLLT) on acute lung inflammation (ALI) induced by lipopolysaccharide (LPS) is linked to tumor necrosis factor (TNF) in alveolar macrophages (AM) from bronchoalveolar lavage fluid (BALF) of mice. LLLT has been reported to actuate positively for relieving the late and early symptoms of airway and lung inflammation. It is not known if the increased TNF mRNA expression and dysfunction of cAMP generation observed in ALI can be influenced by LLLT. for in vivo studies, Balb/c mice (n = 5 for group) received LPS inhalation or TNF intra nasal instillation and 3 h after LPS or TNF-alpha, leukocytes in BALF were analyzed. LLLT administered perpendicularly to a point in the middle of the dissected bronchi with a wavelength of 660 nm and a dose of 4.5 J/cm(2). the mice were irradiated 15 min after ALI induction. in vitro AM from mice were cultured for analyses of TNF mRNA expression and protein and adenosine3':5'-cyclic monophosphate (cAMP) levels. One hour after LPS, the TNF and cAMP levels in AM were measured by ELISA. RT-PCR was used to measure TNF mRNA in AM. the LLLT was inefficient in potentiating the rolipram effect in presence of a TNF synthesis inhibitor. LLLT attenuated the neutrophil influx and TNF in BALF. in AM, the laser increased the cAMP and reduced the TNF-alpha mRNA. LLLT increases indirectly the cAMP in AM by a TNF-dependent mechanism.en
dc.description.affiliationFed Univ São Paulo UNIFESP, Dept Sci & Technol, Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationRes & Dev Inst IP&D, Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationDept Biosyst Engn, BR-36301160 Sao Joao Del Rei, MG, Brazil
dc.description.affiliationUnicastelo, Inst Biomed Engn, BR-12247004 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationCtr Univ Nove Julho Uninove, Rehabil Sci Dept, São Paulo, SP, Brazil
dc.description.affiliationFiocruz MS, Lab Immunopharmacol, BR-21045900 Rio de Janeiro, Brazil
dc.description.affiliationUnifespFed Univ São Paulo UNIFESP, Dept Sci & Technol, Sao Jose Dos Campos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2008/08048-4
dc.description.sponsorshipIDFAPESP: 2007/02596-7
dc.format.extent389-400
dc.identifierhttp://dx.doi.org/10.1007/s10103-010-0874-x
dc.identifier.citationLasers in Medical Science. London: Springer London Ltd, v. 26, n. 3, p. 389-400, 2011.
dc.identifier.doi10.1007/s10103-010-0874-x
dc.identifier.issn0268-8921
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33665
dc.identifier.wosWOS:000289109800016
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofLasers in Medical Science
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectAcute lung inflammationen
dc.subjectAlveolar macrophagesen
dc.subjectCyclic AMPen
dc.subjectTNFen
dc.subjectLow-level laser therapyen
dc.titleLow-level laser therapy (LLLT) acts as cAMP-elevating agent in acute respiratory distress syndromeen
dc.typeinfo:eu-repo/semantics/article
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