Proteomic identification of gender molecular markers in Bothrops jararaca venom

Página do item simplificado
dc.citation.volume139
dc.contributor.authorZelanis, Andre [UNIFESP]
dc.contributor.authorMenezes, Milene C.
dc.contributor.authorKitano, Eduardo S.
dc.contributor.authorLiberato, Tarcisio [UNIFESP]
dc.contributor.authorTashima, Alexandre Keiji [UNIFESP]
dc.contributor.authorPinto, Antonio F. M.
dc.contributor.authorSherman, Nicholas E.
dc.contributor.authorHo, Paulo L.
dc.contributor.authorFox, Jay W.
dc.contributor.authorSerrano, Solange Maria de Toledo [UNIFESP]
dc.coverageAmsterdam
dc.date.accessioned2020-07-22T13:23:06Z
dc.date.available2020-07-22T13:23:06Z
dc.date.issued2016
dc.description.abstractVariation in the snake venom proteome is a well-documented phenomenonen
dc.description.abstracthowever, sex-based variation in the venom proteome/peptidome is poorly understood. Bothrops jararaca shows significant sexual size dimorphism and here we report a comparative proteomic/peptidomic analysis of venoms from male and female specimens and correlate it with the evaluation of important venom features. We demonstrate that adult male and female venoms have distinct profiles of proteolytic activity upon fibrinogen and gelatin. These differences were clearly reflected in their different profiles of SDS-PAGE, two-dimensional electrophoresis and glycosylated proteins. Identification of differential protein bands and spots between male or female venoms revealed gender-specific molecular markers. However, the proteome comparison by in-solution trypsin digestion and label-free quantification analysis showed that the overall profiles of male and female venoms are similar at the polypeptide chain level but show striking variation regarding their attached carbohydrate moieties. The analysis of the peptidomes of male and female venoms revealed different contents of peptides, while the bradykinin potentiating peptides (BPPs) showed rather similar profiles. Furthermore we confirmed the ubiquitous presence of four BPPs that lack the C-terminal Q-I-P-P sequence only in the female venom as gender molecular markers. As a result of these studies we demonstrate that the sexual size dimorphism is associated with differences in the venom proteome/peptidome in B. jararaca species. Moreover, gender-based variations contributed by different glycosylation levels in toxins impact venom complexity. Biological significance: Bothrops jararaca is primarily a nocturnal and generalist snake species, however, it exhibits a notable ontogenetic shift in diet and in venom proteome upon neonate to adult transition. As is common in the Bothrops genus, B. jararaca shows significant sexual dimorphism in snout-vent length and weight, with females being larger than males. This sexual size dimorphism suggests the tendency for female specimens to feed on larger prey, and for male specimens to go on a diet similar to that of juveniles. Variation in the snake venom proteome is a ubiquitous phenomenon occurring at all taxonomic levels. At the intraspecific variation level, the individual contribution to the venom proteome is important but effects contributed by age and feeding habits may also affect the proteome phenotype. Whether sex-based factors play a role in venom variation of a species that shows sexual size dimorphism is poorly known. The use of proteomic strategies supported by transcriptomic data allows a more comprehensive assessment of venom proteomes uncovering components that are gender-specific. (C) 2016 Elsevier B.V. All rights reserved.en
dc.description.affiliationInst Butantan, Ctr Toxins Immune Response & Cell Signaling, Lab Especial Toxinol Aplicada, Av Vital Brasil 1500, BR-05503000 Sao Paulo, SP, Brazil
dc.description.affiliationPontificia Univ Catolica Rio Grande do Sul, Ctr Pesquisas Biol Mol & Func, Inst Nacl Ciencia & Tecnol TB, Porto Alegre, RS, Brazil
dc.description.affiliationUniv Virginia, Sch Med, Dept Microbiol, Charlottesville, VA 22908 USA
dc.description.affiliationInst Butantan, Ctr Biotecnol, Av Vital Brasil 1500, BR-05503000 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Ciencia & Tecnol, Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Bioquim, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Ciencia & Tecnol, Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Bioquim, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico
dc.description.sponsorshipIDFAPESP: 98/14307-9
dc.description.sponsorshipIDFAPESP: 06/60486-0
dc.description.sponsorshipIDFAPESP: 07/54626-7
dc.description.sponsorshipIDFAPESP: 13/07467-1
dc.description.sponsorshipIDFAPESP: 13/13548-4
dc.description.sponsorshipIDCAPES: 1214/2011
dc.description.sponsorshipIDCNPq: 304103/2012-8
dc.format.extent26-37
dc.identifierhttp://dx.doi.org/10.1016/j.jprot.2016.02.030
dc.identifier.citationJournal Of Proteomics. Amsterdam, v. 139, p. 26-37, 2016.
dc.identifier.doi10.1016/j.jprot.2016.02.030
dc.identifier.issn1874-3919
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56034
dc.identifier.wosWOS:000375164600003
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofJournal Of Proteomics
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectBothrops jararaca venomen
dc.subjectMass spectrometryen
dc.subjectPeptidomeen
dc.subjectProteomeen
dc.subjectSexual dimorphismen
dc.subjectSnake venomen
dc.titleProteomic identification of gender molecular markers in Bothrops jararaca venomen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
ICT - Artigos
EPM - Artigos