Effects of Rho-kinase inhibition in lung tissue with chronic inflammation

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dc.contributor.authorRighett, Renato Fraga
dc.contributor.authorSilva Pigati, Patricia Angeli da
dc.contributor.authorPossa, Samantha Souza
dc.contributor.authorHabrum, Fabio Cetinic
dc.contributor.authorXisto, Debora Goncalves
dc.contributor.authorAntunes, Mariana Alves
dc.contributor.authorLeick, Edna Aparecida
dc.contributor.authorPrado, Carla Maximo [UNIFESP]
dc.contributor.authorMartins, Milton de Arruda
dc.contributor.authorMacedo Rocco, Patricia Rieken
dc.contributor.authorLopes Calvo Tiberio, Iolanda de Fatima
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:35:15Z
dc.date.available2016-01-24T14:35:15Z
dc.date.issued2014-02-01
dc.description.abstractWe evaluated whether Rho-kinase inhibition (Y-27632) modulated distal lung responsiveness, inflammation, extracellular matrix remodeling and oxidative stress activation in guinea pigs (GPs) with chronic allergic inflammation. GPs were submitted to inhalation of ovalbumin (OVA-2x/week/4 weeks). From the 5th inhalation on, the Rho-kinase inhibitor group animals were submitted to Y-27632 inhalation 10 min before each inhalation of OVA. Seventy-two hours after the seventh inhalation, the oscillatory mechanics of the distal lung strips were assessed under the baseline condition and after the ovalbumin challenge. Subsequently, the lung slices were submitted to morphometry. Rho-kinase inhibition in the ovalbumin-exposed animals attenuated distal lung elastance and resistance, eosinophils, IL-2, IL-4, IL-5, IL-13, TIMP-1, MMP-9, TGF-beta, IFN-gamma, NF-kappa B and iNOS-positive cells and the volume fraction of 8-iso-PGF2 alpha, elastic, collagen and actin in alveolar walls compared with the OVA group (P < 0.05). Rho-kinase inhibition contributed to the control of distal lung responsiveness, eosinophilic and Th1/Th2 responses and extracellular matrix remodeling in an animal model of chronic allergic inflammation. (C) 2014 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, Sch Med, Dept Med, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Ilha Fundao, Carlos Chagas Filho Inst Biophys, Lab Pulm Invest, Rio de Janeiro, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biol Sci, BR-09972270 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biol Sci, BR-09972270 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent134-146
dc.identifierhttp://dx.doi.org/10.1016/j.resp.2013.12.012
dc.identifier.citationRespiratory Physiology & Neurobiology. Amsterdam: Elsevier B.V., v. 192, p. 134-146, 2014.
dc.identifier.doi10.1016/j.resp.2013.12.012
dc.identifier.issn1569-9048
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37393
dc.identifier.wosWOS:000331673300018
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofRespiratory Physiology & Neurobiology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectY-27632en
dc.subjectRho-kinaseen
dc.subjectInflammationen
dc.subjectDistal lungen
dc.subjectAsthmaen
dc.titleEffects of Rho-kinase inhibition in lung tissue with chronic inflammationen
dc.typeinfo:eu-repo/semantics/article
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