Analysis of the Ontogenetic Variation in the Venom Proteome/Peptidome of Bothrops jararaca Reveals Different Strategies to Deal with Prey

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dc.contributor.authorZelanis, Andre
dc.contributor.authorTashima, Alexandre Keiji [UNIFESP]
dc.contributor.authorRocha, Marisa Maria Teixeira
dc.contributor.authorFurtado, Maria F.
dc.contributor.authorCamargo, Antonio Carlos Martins de
dc.contributor.authorHo, Paulo Lee
dc.contributor.authorSerrano, Solange Maria de Toledo
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:59:39Z
dc.date.available2016-01-24T13:59:39Z
dc.date.issued2010-05-01
dc.description.abstractPrevious studies have demonstrated that the pharmacological activities displayed by Bothrops jararaca venom undergo a significant ontogenetic shift. Variation in the venom proteome is a well-documented phenomenon; however, variation in the venom peptidome is poorly understood. We report a comparative proteomic and peptidomic analysis of venoms from newborn and adult specimens of B. jararaca and correlate it with the evaluation of important venom features. We demonstrate that newborn and adult venoms have similar hemorrhagic activities, while the adult venom has a slightly higher lethal activity in mice; however, the newborn venom is extremely more potent to kill chicks. the coagulant activity of newborn venom upon human plasma is 10 times higher than that of adult venom. These differences were clearly reflected in their different profiles of SDS-PAGE, gelatin zimography, immunostaining using specific antibodies, glycosylation pattern, and concanavalin A-binding proteins. Furthermore, we report for the first time the analysis of the peptide fraction of newborn and adult venoms by MALDI-TOF mass spectrometry and LC-MS/MS, which revealed different contents of peptides, while the bradykinin potentiating peptides (BPPs) showed rather similar profiles and were detected in the venoms showing their canonical sequences and also novel sequences corresponding to BPPs processed from their precursor protein at sites so far not described. As a result of these studies, we demonstrated that the ontogenetic shift in diet, from ectothermic prey in early life to endothermic prey in adulthood, and in animal size are associated with changes in the venom proteome in B. jararaca species.en
dc.description.affiliationInst Butantan, Lab Especial Toxinol Aplicada CAT Cepid, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Quim, Dept Bioquim, BR-05508 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, São Paulo, Brazil
dc.description.affiliationInst Butantan, Lab Herpetol, BR-05503900 São Paulo, Brazil
dc.description.affiliationInst Butantan, Ctr Biotecnnol, BR-05503900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipInstituto Nacional de Ciencia e Tecnologia de Toxinas (INCTTox)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 07/54626-7
dc.description.sponsorshipIDFAPESP: 98/14307-9
dc.format.extent2278-2291
dc.identifierhttps://dx.doi.org/10.1021/pr901027r
dc.identifier.citationJournal of Proteome Research. Washington: Amer Chemical Soc, v. 9, n. 5, p. 2278-2291, 2010.
dc.identifier.doi10.1021/pr901027r
dc.identifier.issn1535-3893
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/32524
dc.identifier.wosWOS:000277353200020
dc.language.isoeng
dc.publisherAmer Chemical Soc
dc.relation.ispartofJournal of Proteome Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectGlycosylationen
dc.subjectOntogenetic venom variationen
dc.subjectPeptidomeen
dc.subjectProteomeen
dc.subjectProteinaseen
dc.subjectSnake venomen
dc.titleAnalysis of the Ontogenetic Variation in the Venom Proteome/Peptidome of Bothrops jararaca Reveals Different Strategies to Deal with Preyen
dc.typeinfo:eu-repo/semantics/article
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