Acute exercise modulates the Foxo1/PGC-1 alpha pathway in the liver of diet-induced obesity rats

dc.contributor.authorRopelle, Eduardo Rochete
dc.contributor.authorPauli, José Rodrigo [UNIFESP]
dc.contributor.authorCintra, Dennys Esper
dc.contributor.authorFrederico, Marisa Jadna Silva
dc.contributor.authorPinho, Ricardo Aurino de
dc.contributor.authorVelloso, Lício Augusto
dc.contributor.authorSouza, Claudio Teodoro de
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Extremo Catarinense
dc.date.accessioned2016-01-24T13:52:28Z
dc.date.available2016-01-24T13:52:28Z
dc.date.issued2009-05-01
dc.description.abstractPGC-1 alpha expression is a tissue-specific regulatory feature that is extremely relevant to diabetes. Several studies have shown that PGC-1 alpha activity is atypically activated in the liver of diabetic rodents and contributes to hepatic glucose production. PGC-1 alpha and Foxo1 can physically interact with one another and represent an important signal transduction pathway that governs the synthesis of glucose in the liver. However, the effect of physical activity on PGC-1 alpha/Foxo1 association is unknown. Here we investigate the expression of PGC-1 alpha and the association of PGC-1 alpha/Foxo1 in the liver of diet-induced obese rats after acute exercise. Wistar rats swam for two 3 h-long bouts, separated by a 45 min rest period. Eight hours after the acute exercise protocol, the rats were submitted to an insulin tolerance test (ITT) and biochemical and molecular analysis. Results demonstrate that acute exercise improved insulin signalling, increasing insulin-stimulated Akt and Foxo1 phosphorylation and decreasing PGC-1 alpha expression and PGC-1 alpha/Foxo1 interaction in the liver of diet-induced obesity rats under fasting conditions. These phenomena are accompanied by a reduction in the expression of gluconeogenesis genes, such as phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphate (G6Pase). Thus, these results provide new insights into the mechanism by which exercise could improve fasting hyperglycaemia.en
dc.description.affiliationUniv Estadual Campinas, Dept Clin Med, FCM, Campinas, SP, Brazil
dc.description.affiliationUNIFESP, Curso Educ Fis Modalidade Saude, Dept Biociencias, Santos, SP, Brazil
dc.description.affiliationUniv Extremo Catarinense, Lab Fisiol & Bioquim Exercicio, Programa Posgrad Ciencias Saude, Criciuma, SC, Brazil
dc.description.affiliationUnifespUNIFESP, Curso Educ Fis Modalidade Saude, Dept Biociencias, Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.format.extent2069-2076
dc.identifierhttps://dx.doi.org/10.1113/jphysiol.2008.164202
dc.identifier.citationJournal of Physiology-london. Malden: Wiley-Blackwell Publishing, Inc, v. 587, n. 9, p. 2069-2076, 2009.
dc.identifier.doi10.1113/jphysiol.2008.164202
dc.identifier.issn0022-3751
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/31478
dc.identifier.wosWOS:000265617000022
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Physiology-london
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.titleAcute exercise modulates the Foxo1/PGC-1 alpha pathway in the liver of diet-induced obesity ratsen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções