Effects of carbachol on rat Sertoli cell proliferation and muscarinic acetylcholine receptors regulation: an in vitro study

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dc.contributor.authorLucas, TFG
dc.contributor.authorAvellar, MCW
dc.contributor.authorPorto, C. S.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:37:19Z
dc.date.available2016-01-24T12:37:19Z
dc.date.issued2004-08-20
dc.description.abstractThe aim of the present work was to study the effect of muscarinic agonist on cell proliferation and muscarinic acetylcholine receptors (mAChRs) regulation in rat Sertoli cells. Primary cultures of Sertoli cells were obtained from 8-day and, 15-day old male Wistar rats. in proliferation assays, [methyl-H-3]thymidine incorporation in Sertoli cells from 8-day and 15-day old rats reached a plateau after 60 min of carbachol incubation and decreased after 120 min of agonist incubation. Binding studies with [N-Methyl-H-3]scopolamine ([H-3]NMS) indicated a rapid loss of cell surface mAChRs when Sertoli cells from 15-day old rats were incubated with carbachol at 35 degreesC for 2 min. This effect was temperature-dependent. When the incubation of the cells was prolonged at 35 degreesC or at 4 degreesC, after the agonist had been washed away, 94% of mAChRs were present in the cell surface after 120 min incubation at 35 degreesC. At 4 degreesC, however, a low percentage of mAChRs was detected in the cell surface. in the presence of cycloheximide, the recycling of mAChRs to the cell surface was not changed, suggesting that the appearance of mAChRs on cell surface was not dependent on de novo receptor synthesis. in conclusion, our studies indicate that the activation of mAChRs may play a role in rat Sertoli cell proliferation. These receptors may be under regulation (internalization and recycling) when cells are exposed to muscarinic cholinergic agonist. (C) 2004 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol,INFAR, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol,INFAR, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1761-1773
dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2004.05.006
dc.identifier.citationLife Sciences. Oxford: Pergamon-Elsevier B.V., v. 75, n. 14, p. 1761-1773, 2004.
dc.identifier.doi10.1016/j.lfs.2004.05.006
dc.identifier.issn0024-3205
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27888
dc.identifier.wosWOS:000223012100007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofLife Sciences
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectmuscarinic receptorsen
dc.subjectcarbacholen
dc.subjectcell proliferation (Sertoli cells)en
dc.subjectinternalization and recycling of receptorsen
dc.titleEffects of carbachol on rat Sertoli cell proliferation and muscarinic acetylcholine receptors regulation: an in vitro studyen
dc.typeinfo:eu-repo/semantics/article
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