Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract

dc.contributor.authorPatrão, Marilia Tavares Coutinho da Costa [UNIFESP]
dc.contributor.authorQueiroz, Daniel Barboza Cava [UNIFESP]
dc.contributor.authorGrossman, Gail
dc.contributor.authorPetrusz, Peter
dc.contributor.authorLazari, Maria de Fatima Magalhaes [UNIFESP]
dc.contributor.authorAvellar, Maria Christina Werneck [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv N Carolina
dc.date.accessioned2016-01-24T13:49:30Z
dc.date.available2016-01-24T13:49:30Z
dc.date.issued2008-02-01
dc.description.abstractThis study reports the genomic organization of the rhesus alpha(1A)-adrenoceptor gene (ADRA1A). Full-length cloning of rhesus ADRA1A splice variants was achieved by combining PCR screening of a seminal vesicle cDNA library and 5'-RACE assays with total RNA from seminal vesicle. the classical ADRA1A mRNA (ADRA1A_nu 1) and six full-length ADRA1A splice variants were identified representing transcripts that code for functional (ADRA1A_nu 1, ADRA1A_nu 2a, ADRA1A_nu 3a, ADRA1A_nu 3d, ADRA1A_nu 3e) and truncated (ADRA1A_nu 2c and ADRA1A_nu 3c) receptor isoforms. Comparative analysis of the deduced amino acid sequence indicated that rhesus ADRA1A_i1 isoform (corresponding to the ADRA1A_nu 1 transcript) shares high identity to the amino acid sequence present in the classical alpha(1A)-adrenoceptor from human and other mammalian species. Partial nucleotide sequences for rhesus alpha(1B)-(ADRA1B) and alpha(1D)-adrenoceptor (ADRA1D) transcripts were also characterized. RT-PCR studies indicated differential distribution of all ADRA1A-related splice variants as well as ADRA1B and ADRA1D mRNAs, in tissues from rhesus and human male reproductive tract. Immunohistochemistry revealed alpha(1A)-adrenoceptor (ADRA1A_ i1) immunostaining in smooth muscle cells and epithelial cells of rhesus efferent ductules, epididymis and seminal vesicle. Taken together the present results demonstrate that the complexity of the splicing mechanisms involved in the regulation of the ADRA1A gene is not restricted to human and is a common characteristic among Old World monkeys.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, Escola Paulista Med,INFAR, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv N Carolina, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USA
dc.description.affiliationUniv N Carolina, Labs Reprod Biol, Chapel Hill, NC 27599 USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, Escola Paulista Med,INFAR, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent85-96
dc.identifierhttps://dx.doi.org/10.1093/molehr/gam084
dc.identifier.citationMolecular Human Reproduction. Oxford: Oxford Univ Press, v. 14, n. 2, p. 85-96, 2008.
dc.identifier.doi10.1093/molehr/gam084
dc.identifier.issn1360-9947
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/30391
dc.identifier.wosWOS:000257148600003
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofMolecular Human Reproduction
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.subjectalpha(1)-adrenoceptoren
dc.subjectHumanen
dc.subjectMale reproductive tracten
dc.subjectRhesus monkeyen
dc.subjectSplice variantsen
dc.titleCloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tracten
dc.typeinfo:eu-repo/semantics/article
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