hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions

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dc.contributor.authorSilva, Tanielly Cristina Raiol
dc.contributor.authorLeal, Mariana Ferreira [UNIFESP]
dc.contributor.authorCalcagno, Danielle Queiroz [UNIFESP]
dc.contributor.authorSouza, Carolina Rosal Teixeira de
dc.contributor.authorKhayat, Andre Salim
dc.contributor.authorSantos, Ney Pereira Carneiro dos
dc.contributor.authorMontenegro, Raquel Carvalho
dc.contributor.authorRabenhorst, Silvia Helena Barem
dc.contributor.authorNascimento, Mayara Quaresma
dc.contributor.authorAssumpcao, Paulo Pimentel
dc.contributor.authorSmith, Marilia de Arruda Cardoso [UNIFESP]
dc.contributor.authorBurbano, Rommel Rodriguez
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFed Univ Para
dc.contributor.institutionUniv Fed Ceara
dc.date.accessioned2016-01-24T14:27:28Z
dc.date.available2016-01-24T14:27:28Z
dc.date.issued2012-07-06
dc.description.abstractBackground: Gastric cancer is a serious public health problem in Northern Brazil and in the world due to its high incidence and mortality. Despite the severity of the disease, more research is needed to better understand the molecular events involved in this intestinal-type gastric carcinogenesis process. Since precancerous lesions precede intestinal-type gastric cancer, here, we evaluated the hTERT, MYC, and TP53 mRNA and protein expression, as well as TP33 copy number, in gastric preneoplastic lesions.Methods: We evaluated 19 superficial gastritis, 18 atrophic gastritis, and 18 intestinal metaplasia from cancer-free individuals of Northern Brazil. Quantitative reverse transcription PCR was used to analyze the mRNA expression and immunohistochemical methods were used to assess protein immunoreactivity in tissue samples. the number of TP53 gene copies was investigated in gastric diseases by quantitative PCR.Results: We observed hTERT, MYC, and p53 immunoreactivity only in intestinal metaplasia samples. the immunoreactivity of these proteins was strongly associated with each other. A significantly higher MYC mRNA expression was observed in intestinal metaplasia compared to gastritis samples. Loss of TP53 was also only detected in intestinal metaplasia specimens.Conclusions: We demonstrated that hTERT, MYC, and TP53 are deregulated in intestinal metaplasia of individuals from Northern Brazil and these alterations may facilitate tumor initiation.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Genet, BR-04023900 São Paulo, Brazil
dc.description.affiliationFed Univ Para, Inst Ciencias Biol, Lab Citogenet Humana, BR-66073000 Belem, PA, Brazil
dc.description.affiliationFed Univ Para, Hosp Univ Joao de Barros Barreto, Unidade Alta Complexidade Oncol, BR-60673000 Belem, PA, Brazil
dc.description.affiliationUniv Fed Ceara, Escola Med, Dept Patol & Med Forense, Genet Mol Lab, BR-60020181 Fortaleza, CE, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Genet, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDCNPq: 302774/2009-2
dc.description.sponsorshipIDCNPq: 301609/2007-1
dc.format.extent8
dc.identifierhttp://dx.doi.org/10.1186/1471-230X-12-85
dc.identifier.citationBmc Gastroenterology. London: Biomed Central Ltd, v. 12, 8 p., 2012.
dc.identifier.doi10.1186/1471-230X-12-85
dc.identifier.fileWOS000310335300001.pdf
dc.identifier.issn1471-230X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35090
dc.identifier.wosWOS:000310335300001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofBmc Gastroenterology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjecthTERTen
dc.subjectMYCen
dc.subjectTP53en
dc.subjectGastric carcinogenesisen
dc.subjectPrecancerous lesionsen
dc.titlehTERT, MYC and TP53 deregulation in gastric preneoplastic lesionsen
dc.typeinfo:eu-repo/semantics/article
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