Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture

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dc.citation.issue12
dc.citation.volume7
dc.contributor.authorValdetaro, Gisele P.
dc.contributor.authorAldrovani, Marcela
dc.contributor.authorPadua, Ivan R. M.
dc.contributor.authorCristovam, Priscila Cardoso [UNIFESP]
dc.contributor.authorGomes, José Álvaro Pereira [UNIFESP]
dc.contributor.authorLaus, Jose L.
dc.coverageWashington
dc.date.accessioned2020-07-31T12:47:09Z
dc.date.available2020-07-31T12:47:09Z
dc.date.issued2016
dc.description.abstractIn this research we evaluated the supramolecular organizations and the optical anisotropical properties of the de-epithelialized human amniotic membrane and rabbit limbal stroma, before and after explant culture. Birefringence, monochromatic light spectral absorption and linear dichroism of the main extracellular matrix biopolymers, that is, the fibrillar collagens and proteoglycans, were investigated by polarized light microscopy combined with image analysis. Our results demonstrated that the culture procedure-induced stimuli altered the supra-organizational characteristics (in terms of collagens/proteoglycans spatial orientation and ordered-aggregational state) of the amniotic and limbal extracellular matrix, which led to changes in optical anisotropical properties. (C)2016 Optical Society of Americaen
dc.description.affiliationUNESP Jaboticabal, Ophthalmol Unit, Dept Small Anim Med & Surg, Fac Agr & Vet Sci, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, UNIFESP Sao Paulo, Ocular Surface Adv Ctr, BR-04039002 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespOcular Surface Advanced Center, Universidade Federal de São Paulo (UNIFESP), São Paulo, 04039-002, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSao Paulo Research Foundation - FAPESP [2012/17308-5, 2013/01494-7, 2013/25533-1]
dc.description.sponsorshipNational Council for Scientific and Technological Development - CNPq [467289/2014-0]
dc.description.sponsorshipIDSao Paulo Research Foundation - FAPESP [2012/17308-5, 2013/01494-7, 2013/25533-1]
dc.description.sponsorshipIDNational Council for Scientific and Technological Development - CNPq [467289/2014-0]
dc.format.extent4982-4994
dc.identifierhttp://dx.doi.org/10.1364/BOE.7.004982
dc.identifier.citationBiomedical Optics Express. Washington, v. 7, n. 12, p. 4982-4994, 2016.
dc.identifier.doi10.1364/BOE.7.004982
dc.identifier.fileWOS000390490700012.pdf
dc.identifier.issn2156-7085
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56620
dc.identifier.wosWOS:000390490700012
dc.language.isoeng
dc.publisherOptical Soc Amer
dc.relation.ispartofBiomedical Optics Express
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleSupra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant cultureen
dc.typeinfo:eu-repo/semantics/article
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