Blood flow measurements in rats using four color microspheres during blockade of different vasopressor systems

Página do item simplificado
dc.contributor.authorDe Angelis, Katia
dc.contributor.authorGama, V. M. [UNIFESP]
dc.contributor.authorFarah, VAM
dc.contributor.authorIrigoyen, Maria Claudia [UNIFESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Santo Amaro
dc.contributor.institutionUniv Sao Judas Tadeu
dc.date.accessioned2016-01-24T12:37:32Z
dc.date.available2016-01-24T12:37:32Z
dc.date.issued2005-01-01
dc.description.abstractThe use of colored microspheres to adequately evaluate blood flow chancres under different circumstances in the same rat has been validated with a maximum of three different colors due to methodological limitations. the aim of the present study was to validate the use of four different colors measuring four repeated blood flow. changes in the same rat to assess the role of vasopressor systems in. controlling arterial pressure (AP). Red (150,000), white (200,000)), yellow (150,000), and blue (200,000) colored microspheres were infused into the left ventricle of 6 male Wistar rats 1) at rest and 2) after vasopressin (aAVP, 10 mug/kg, iv), 3) renin-angiotensin (losartan, 10 ms/kg iv), and 4) sympathetic system blockade (hexamethonium., 20 mg/kg, iv) to determine blood flow changes. AP was recorded and processed with a data acquisition system (1-kHz sampling frequency). Blood flow changes were quantified by spectrophotometry absorption peaks for colored microsphere components in the tissues evaluated. Administration of aAVP and losartan slightly reduced the AP (-5.7 +/- 0.5 and -7.8 +/- 1.2 mmHg, respectively), while hexamethonium induced a 52 +/- 3 mmHg fall in AP. the aAVP injection increased blood flow in lungs (78%), liver (117%) and skeletal muscle (>150%), while losartan administration enhanced blood flow in heart (126%), lungs (100%), kidneys (80%), and gastrocnemius (75%) and soleus (94%) muscles. Hexamethonium administration reduced only kidney blood flow (50%). in conclusion, four types of colored microspheres can be used to perform four repeated blood flow measurements in the same rat detecting small alterations such as changes in tissues with low blood flow.en
dc.description.affiliationUniv São Paulo, Fac Med, Inst Coracao, Unidade Hipertensao, BR-05403000 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Nefrol, São Paulo, Brazil
dc.description.affiliationUniv Santo Amaro, UNIPESQ, Santo Amaro, SP, Brazil
dc.description.affiliationUniv Sao Judas Tadeu, Lab Movimiento Humano, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Nefrol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent119-125
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2005000100018
dc.identifier.citationBrazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 38, n. 1, p. 119-125, 2005.
dc.identifier.doi10.1590/S0100-879X2005000100018
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2005000100018
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28067
dc.identifier.wosWOS:000226663600018
dc.language.isoeng
dc.publisherAssoc Bras Divulg Cientifica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcolored microspheresen
dc.subjectarterial pressureen
dc.subjectsympathetic systemen
dc.subjectrenin-angiotensinen
dc.subjectvasopressinen
dc.subjectlocal blood flow measurementen
dc.titleBlood flow measurements in rats using four color microspheres during blockade of different vasopressor systemsen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
S0100-879X2005000100018.pdf
Tamanho:
504.15 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
EPM - Artigos