A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi

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dc.contributor.authorSuzuki, Erika [UNIFESP]
dc.contributor.authorToledo, Marcos Sergio de [UNIFESP]
dc.contributor.authorTakahashi, Helio Kiyoshi [UNIFESP]
dc.contributor.authorStraus, Anita Hilda [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:30:21Z
dc.date.available2016-01-24T12:30:21Z
dc.date.issued1997-06-01
dc.description.abstractA mouse monoclonal antibody, MEST-1, was produced against Band 1 glycolipid antigen of Paracoccidioides brasiliensis, the glycan structure of Band 1 antigen was recently elucidated and the monosaccharides sequence was defined as: Galf beta 1-->6(Manp alpha 1-->3)Manp beta 1-->2Ins. the reactivity of MEST-1 MAb was determined by solid-phase radioimmunoassay and high performance thin layer chromatography immunostaining. Selective oxidation of galactofuranose residues and inhibition assays with different methyl-glycosides, revealed that MAb MEST-1 is directed against the terminal residue of beta-D-gaiactofuranose of Band 1, a phosphoglyceroglycolipid antigen of P.brasiliensis. By indirect immunofluorescence, it was observed that the epitope recognized by MEST-1 is accessible to the antibody in yeast forms of this fungus. Reactivity of MEST-1 with parasites known to express galactofuranose containing glycoconjugates was also analyzed by indirect immunofluorescence. A positive fluorescence was observed with promastigotes of Leishmania major and epimastigotes of Trypanosoma cruzi, GIPL-1 was identified as the antigen recognized by MEST-1 in Leishmania major, indicating that the MAb MEST-1 recognizes terminal galactofuranose residue in either beta 1-->6 or beta 1-->3 linkage to the mannose.en
dc.description.affiliationUniversidade Federal de São Paulo,DEPT BIOCHEM,EPM,BR-04023900 São Paulo,BRAZIL
dc.description.affiliationUnifespUniversidade Federal de São Paulo,DEPT BIOCHEM,EPM,BR-04023900 São Paulo,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent463-468
dc.identifierhttp://dx.doi.org/10.1093/glycob/7.4.463
dc.identifier.citationGlycobiology. Oxford: Oxford Univ Press, v. 7, n. 4, p. 463-468, 1997.
dc.identifier.doi10.1093/glycob/7.4.463
dc.identifier.issn0959-6658
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/25729
dc.identifier.wosWOS:A1997XB69300003
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofGlycobiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.subjectgalactofuranoseen
dc.subjectglycolipid antigenen
dc.subjectmonoclonal antibodyen
dc.subjectParacoccidioides brasiliensisen
dc.subjectLeishmania majoren
dc.titleA monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzien
dc.typeinfo:eu-repo/semantics/article
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