Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in mice

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dc.contributor.authorBivi, Nicoletta
dc.contributor.authorCosta, Rafael Pacheco da [UNIFESP]
dc.contributor.authorBrun, Lucas R.
dc.contributor.authorMurphy, Thomas R.
dc.contributor.authorFarlow, Nathan R.
dc.contributor.authorRobling, Alexander G.
dc.contributor.authorBellido, Teresita
dc.contributor.authorPlotkin, Lilian I.
dc.contributor.institutionIndiana Univ Sch Med
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionRichard L Roudebush Vet Adm Med Ctr
dc.date.accessioned2016-01-24T14:31:53Z
dc.date.available2016-01-24T14:31:53Z
dc.date.issued2013-07-01
dc.description.abstractThe osteocyte network is crucial for the response of bone to mechanical force. Within this network, connexin43 (Cx43) is thought to mediate the communication of osteocytes and osteoblasts among themselves and the exchange of small molecules with the extracellular milieu. Despite recent advances in understanding Cx43 role for the response of bone cells to mechanical stimulation, the contribution of Cx43 specifically in osteocytes to mechanotransduction in vivo is not well-known. We examined the anabolic response to ulnar axial loading of mice lacking Cx43 in osteocytes (Cx43Ot). Loading induced a greater increase in periosteal bone formation rate in Cx43Ot mice compared to control littermates, resulting from higher mineralizing surface and enhanced mineral apposition rate. Expression of -catenin protein, a molecule implicated in mechanotransduction, was higher in bones from Cx43Ot mice, compared to littermate controls. in addition, MLO-Y4 osteocytic cells knocked-down for Cx43 exhibited higher -catenin protein expression and enhanced response to mechanical stimulation. These findings suggest that osteocytes lacking Cx43 are primed to respond to mechanical stimulation and that absence of Cx43 in osteocytes unleashes bone formation, by a mechanism that might involve accumulation of -catenin. (c) 2013 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 31:10751081, 2013en
dc.description.affiliationIndiana Univ Sch Med, Dept Anat & Cell Biol, Indianapolis, in USA
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morphol & Genet, São Paulo, Brazil
dc.description.affiliationIndiana Univ Sch Med, Dept Internal Med, Div Endocrinol, Indianapolis, in USA
dc.description.affiliationRichard L Roudebush Vet Adm Med Ctr, Indianapolis, in 46202 USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Morphol & Genet, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipNIH
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDNIH: R01-AR053643
dc.description.sponsorshipIDCAPES: 1065/11-4
dc.format.extent1075-1081
dc.identifierhttp://dx.doi.org/10.1002/jor.22341
dc.identifier.citationJournal of Orthopaedic Research. Hoboken: Wiley-Blackwell, v. 31, n. 7, p. 1075-1081, 2013.
dc.identifier.doi10.1002/jor.22341
dc.identifier.issn0736-0266
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36437
dc.identifier.wosWOS:000319350500012
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Orthopaedic Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectconnexin43en
dc.subjectbone formationen
dc.subjectmechanical loadingen
dc.subjectosteocyteen
dc.subjectbeta-cateninen
dc.titleAbsence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in miceen
dc.typeinfo:eu-repo/semantics/article
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