TccP2-mediated subversion of actin dynamics by EPEC 2 - a distinct evolutionary lineage of enteropathogenic Escherichia coli

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dc.contributor.authorWhale, Andrew D.
dc.contributor.authorHernandes, Rodrigo T. [UNIFESP]
dc.contributor.authorOoka, Tadasuke
dc.contributor.authorBeutin, Lothar
dc.contributor.authorSchuller, Stephanie
dc.contributor.authorGarmendia, Junkal
dc.contributor.authorCrowther, Lynette
dc.contributor.authorVieira, Mônica Aparecida Midolli [UNIFESP]
dc.contributor.authorOgura, Yoshitoshi
dc.contributor.authorKrause, Gladys
dc.contributor.authorIllips, Alan D. Ph
dc.contributor.authorGomes, Tania A. T.
dc.contributor.authorHayashi, Tetsuya
dc.contributor.authorFrankel, Gad
dc.contributor.institutionUniv London Imperial Coll Sci Technol & Med
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionMiyazaki Univ
dc.contributor.institutionBundesinst Risikobewertung
dc.contributor.institutionUCL Royal Free & Univ Coll Med Sch
dc.contributor.institutionRecinto Hosp Joan March
dc.date.accessioned2016-01-24T13:48:45Z
dc.date.available2016-01-24T13:48:45Z
dc.date.issued2007-06-01
dc.description.abstractEnteropathogenic Escherichia coli (EPEC) is a major cause of infantile diarrhoea in developing countries. While colonizing the gut mucosa, EPEC triggers extensive actin-polymerization activity at the site of intimate bacterial attachment, which is mediated by avid interaction between the outer-membrane adhesin intimin and the type III secretion system (T3SS) effector Tir. the prevailing dogma is that actin polymerization by EPEC is achieved following tyrosine phosphorylation of Tir, recruitment of Nck and activation of neuronal Wiskott-Aldrich syndrome protein (N-WASP). in closely related enterohaemorrhagic E. coli (EHEC) O157 : H7, actin polymerization is triggered following recruitment of the T3SS effector TccP/EspF(U) (instead of Nck) and local activation of N-WASP. in addition to tccP, typical EHEC O157 : H7 harbour a pseudogene (tccP2). However, it has recently been found that atypical, sorbitol-fermenting EHEC O157 carries functional tccP and tccP2 alleles. Interestingly, intact tccP2 has been identified in the incomplete genome sequence of the prototype EPEC strain B171 (serotype O111 : H-), but it is missing from another prototype EPEC strain E2348/69 (O127 : H7). E2348/69 and B171 belong to two distinct evolutionary lineages of EPEC, termed EPEC 1 and EPEC 2, respectively. Here, it is reported that while both EPEC 1 and EPEC 2 triggered actin polymerization via the Nck pathway, tccP2 was found in 26 of 27 (96.2%) strains belonging to EPEC 2, and in none of the 34 strains belonging to EPEC 1. It was shown that TccP2 was: (i) translocated by the locus of enterocyte effacement-encoded T3SS; (ii) localized at the tip of the EPEC 2-induced actin-rich pedestals in infected HeLa cells and human intestinal in vitro organ cultures ex vivo; and (iii) essential for actin polymerization in infected Nck-/- cells. Therefore, unlike strains belonging to EPEC 1, strains belonging to EPEC 2 can trigger actin polymerization using both Nck and TccP2 actin-polymerization signalling cascades.en
dc.description.affiliationUniv London Imperial Coll Sci Technol & Med, Div Cell & Mol Biol, London SW7 2AZ, England
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationMiyazaki Univ, Frontier Sci Res Ctr, Div Bioenvironm Sci, Miyazaki 8891692, Japan
dc.description.affiliationBundesinst Risikobewertung, Natl Referenzlab Escherichia Coli, D-12277 Berlin, Germany
dc.description.affiliationUCL Royal Free & Univ Coll Med Sch, Ctr Paediat Gastroenterol, London, England
dc.description.affiliationRecinto Hosp Joan March, Fdn Caubet Cimera, Bunyola, Mallorca, Spain
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1743-1755
dc.identifierhttp://dx.doi.org/10.1099/mic.0.2006/004325-0
dc.identifier.citationMicrobiology-sgm. Reading: Soc General Microbiology, v. 153, p. 1743-1755, 2007.
dc.identifier.doi10.1099/mic.0.2006/004325-0
dc.identifier.fileWOS000247304900007.pdf
dc.identifier.issn1350-0872
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29767
dc.identifier.wosWOS:000247304900007
dc.language.isoeng
dc.publisherSoc General Microbiology
dc.relation.ispartofMicrobiology-sgm
dc.rightsAcesso aberto
dc.titleTccP2-mediated subversion of actin dynamics by EPEC 2 - a distinct evolutionary lineage of enteropathogenic Escherichia colien
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