Histochemical and ultrastructural analysis of hepatic glycogen and collagen fibers in alloxan-induced diabetic rats submitted to long-term physical training

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dc.contributor.authorRemedio, Rafael Neodini
dc.contributor.authorBarbosa, Rodrigo Avelaira
dc.contributor.authorCastellar, Alexandre
dc.contributor.authorGomes, Ricardo José [UNIFESP]
dc.contributor.authorCaetano, Flavio Henrique
dc.contributor.institutionSão Paulo State Univ UNESP
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:17:03Z
dc.date.available2016-01-24T14:17:03Z
dc.date.issued2011-08-01
dc.description.abstractAlterations in liver functions are common among diabetic patients, and many symptoms in the liver have been reported, including changes in glycogen stores and in the amount of collagen fibers. the practice of physical training and its morphological effects in this organ, however, are scarcely studied. in order to observe the morphological effects of alloxan-induced diabetes and the alterations arising from the practice of long-term chronic physical training in the liver, samples were collected and processed, and then analyzed by means of the histochemical techniques Periodic Acid-Schiff and Picrosirius-Hematoxylin, and ultrastructural cytochemical test of Afzelius. Through evaluation of the tissue, it was observed a drastic reduction in hepatic glycogen stores of sedentary diabetics, recovered in trained diabetic rats. Furthermore, it was detected a decrease in the content of perisinusoidal collagen fibers in the diabetic liver, also recovered due to the development of a training protocol. On ultrastructural level, cytochemical analysis confirmed the loss of glycogen and the recovery obtained by training. in conclusion, the practice of a long-term chronic physical training protocol may be considered an important assistant in the treatment of diabetes, mitigating the occurrence of possible damages to liver tissue. (C) 2011 Elsevier B.V. All rights reserved.en
dc.description.affiliationSão Paulo State Univ UNESP, Dept Biol, Rio Claro, SP, Brazil
dc.description.affiliationSão Paulo Fed Univ UNIFESP, Dept Biosci, Santos, SP, Brazil
dc.description.affiliationUnifespSão Paulo Fed Univ UNIFESP, Dept Biosci, Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent207-215
dc.identifierhttps://dx.doi.org/10.1016/j.tice.2011.03.003
dc.identifier.citationTissue & Cell. Edinburgh: Churchill Livingstone, v. 43, n. 4, p. 207-215, 2011.
dc.identifier.doi10.1016/j.tice.2011.03.003
dc.identifier.issn0040-8166
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/33932
dc.identifier.wosWOS:000208738500001
dc.language.isoeng
dc.publisherChurchill Livingstone
dc.relation.ispartofTissue & Cell
dc.rightsAcesso restrito
dc.subjectDiabetesen
dc.subjectPhysical trainingen
dc.subjectLiveren
dc.subjectHistochemistryen
dc.subjectUltrastructureen
dc.titleHistochemical and ultrastructural analysis of hepatic glycogen and collagen fibers in alloxan-induced diabetic rats submitted to long-term physical trainingen
dc.typeArtigo
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