Impact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of rats

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dc.contributor.authorKracochansky, Mirian [UNIFESP]
dc.contributor.authorReis, Leonardo Oliveira
dc.contributor.authorLorenzetti, Fábio [UNIFESP]
dc.contributor.authorOrtiz, Valdemar [UNIFESP]
dc.contributor.authorDambros, Miriam [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2015-06-14T13:43:42Z
dc.date.available2015-06-14T13:43:42Z
dc.date.issued2012-04-01
dc.description.abstractOBJECTIVE: To analyze the impact of low levels of testosterone induced by orchiectomy and the effect of alpha-tocopherol supplementation on oxidative stress in the urethral sphincter. MATERIALS AND METHODS: Forty male Wistar rats weighing 250-300g were divided into four groups with 10 each: Sham group; Orchiectomy group: bilateral orchiectomy; Orchiectomy-pre-Tocopherol group: bilateral orchiectomy preceded by alpha-tocopherol supplementation for four weeks; Orchiectomy-full-Tocopherol group: bilateral orchiectomy with alpha-tocopherol supplementation for four weeks preceding the procedure and for eight weeks afterwards. At the protocol end, animals were euthanized and had the sphincter analyzed stereologically focusing on collagen and muscle fibers percentage. Oxidative stress levels were determined using 8-epi-PGF2. RESULTS: The 8-epi-PGF2 levels were statistically higher (p < 0.0003) in the Orchiectomy group compared to others groups while Sham and Orchiectomy-full-Tocopherol groups presented statistically similar values (p = 0.52). Collagen volumetric densities were significantly lower in Sham and Orchiectomy-full-Tocopherol groups (p < 0.022). Sham group presented statistically greater muscle fiber percent. CONCLUSION: Castration caused oxidative stress in the urethral sphincter complex, with increased collagen deposition. Alpha-tocopherol had a protective effect and its supplementation for twelve weeks provided the greatest protection.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Division of Geriatric Urology
dc.description.affiliationUniversity of Campinas School of Medical Sciences Division of Urology
dc.description.affiliationUnifespUNIFESP, EPM, Division of Geriatric Urology
dc.description.sourceSciELO
dc.format.extent277-283
dc.identifierhttp://dx.doi.org/10.1590/S1677-55382012000200017
dc.identifier.citationInternational braz j urol. Sociedade Brasileira de Urologia, v. 38, n. 2, p. 277-283, 2012.
dc.identifier.doi10.1590/S1677-55382012000200017
dc.identifier.fileS1677-55382012000200017.pdf
dc.identifier.issn1677-5538
dc.identifier.scieloS1677-55382012000200017
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/7056
dc.identifier.wosWOS:000306550800033
dc.language.isoeng
dc.publisherSociedade Brasileira de Urologia
dc.relation.ispartofInternational braz j urol
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectapoptosisen
dc.subjectalpha-tocopherolen
dc.subjectoxidative stressen
dc.subjectantioxidantsen
dc.subjectcastrationen
dc.titleImpact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of ratsen
dc.typeinfo:eu-repo/semantics/article
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