Ethyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitis

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dc.contributor.authorMatone, Jacques [UNIFESP]
dc.contributor.authorMoretti, Ana Iochabel Soares
dc.contributor.authorApodaca-Torrez, Franz Robert [UNIFESP]
dc.contributor.authorGoldenberg, Alberto [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-06-14T13:45:34Z
dc.date.available2015-06-14T13:45:34Z
dc.date.issued2013-08-01
dc.description.abstractPURPOSE: To investigate if the ethyl-pyruvate solution could reduce mortality in AP and/or diminish the acute lung injury. METHODS: Forty male rats, weighing between 270 to 330 grams were operated. An experimental model of severe AP by injection of 0.1ml/100g of 2.5% sodium taurocholate into the bilio-pancreatic duct was utilized. The rats were divided into two groups of ten animals each: CT - control (treatment with 50ml/kg of Ringer's solution, intraperitoneal) and EP (treatment with 50ml/kg of Ringer ethyl- pyruvate solution, intra-peritoneal), three hours following AP induction. After six hours, a new infusion of the treatment solution was performed in each group. Two hours later, the animals were killed and the pulmonary parenchyma was resected for biomolecular analysis, consisting of: interleukin, myeloperoxidase, MDA, nitric oxide, metalloproteinases and heat shock protein. In the second part of the experiment, another, 20 rats were randomly divided into EP and CT groups, in order to evaluate a survival comparison between the two groups. RESULTS: There were no significant differences in IL-1B,IL-10, MMP-9, HSP70, nitric oxide, MPO, MDA (lipidic peroxidation) concerning both groups. The levels of IL-6 were significantly diminished in the EP group. Furthermore, the MMP-2 levels were also reduced in the EP group (p<0.05). The animals from the EP treatment groups had improved survival, when compared to control group (p<0.05). CONCLUSION: The ethyl-pyruvate diminishes acute lung injury inflammatory response in acute pancreatitis and ameliorates survival when compared to control group, in the experimental model of necrotizing acute pancreatitis.en
dc.description.affiliationUNIFESP-EPM Postgraduate Program in Gastrointestinal Surgery
dc.description.affiliationUSP Department of Emergency Medicine
dc.description.affiliationUNIFESP-EPM Department of Surgery
dc.description.affiliationUnifespUNIFESP, EPM, Postgraduate Program in Gastrointestinal Surgery
dc.description.affiliationUnifespUNIFESP, EPM Department of Surgery
dc.description.sourceSciELO
dc.format.extent559-567
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502013000800002
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 8, p. 559-567, 2013.
dc.identifier.doi10.1590/S0102-86502013000800002
dc.identifier.fileS0102-86502013000800002.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502013000800002
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/7890
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPancreatitisen
dc.subjectAcute Necrotizingen
dc.subjectPyruvic Aciden
dc.subjectInflammationen
dc.subjectRatsen
dc.titleEthyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitisen
dc.typeinfo:eu-repo/semantics/article
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