Treatment of Leishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-Killed Propionibacterium acnes Triggers Protective Cellular Immune Responses

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dc.citation.volume8
dc.contributor.authorPaladi, Carolina S. [UNIFESP]
dc.contributor.authorda Silva, Danielle A. M. [UNIFESP]
dc.contributor.authorMotta, Priscila D. [UNIFESP]
dc.contributor.authorGarcia, Daniel M. [UNIFESP]
dc.contributor.authorTeixeira, Daniela [UNIFESP]
dc.contributor.authorLongo-Maugeri, Ieda M. [UNIFESP]
dc.contributor.authorKatz, Simone [UNIFESP]
dc.contributor.authorBarbieri, Clara L. [UNIFESP]
dc.coverageLausanne
dc.date.accessioned2020-07-17T14:02:40Z
dc.date.available2020-07-17T14:02:40Z
dc.date.issued2017
dc.description.abstractPalladacycle complex DPPE 1.2 was previously reported to inhibit the in vitro and in vivo infection by Leishmania (Leishmania) amazonensis. The aim of the present study was to compare the effect of DPPE 1.2, in association with heat-killed Propionibacterium acnes, on L. (L.) amazonensis infection in two mouse strains, BALB/c and C57BL/6, and to evaluate the immune responses of the treated animals. Foot lesions of L. (L.) amazonensis-infected mice were injected with DPPE 1.2 alone, or associated with P. acnes as an adjuvant. Analysis of T-cell populations in the treated mice and in untreated controls was performed by FACS. Detection of IFN-gamma-secreting lymphocytes was carried out by an ELISPOT assay and active TGF-beta was measured by means of a double-sandwich ELISA test. The treatment with DPPE 1.2 resulted in a significant reduction of foot lesion sizes and parasite burdens in both mouse strains, and the lowest parasite burden was found in mice treated with DPPE 1.2 plus P. acnes. Mice treated with DPPE 1.2 alone displayed a significant increase of TCD4(+) and TCD8(+) lymphocytes and IFN-gamma secretion which were significantly higher in animals treated with DPPE 1.2 plus P. acnes. A significant reduction of active TGF-b was observed in mice treated with DPPE 1.2 alone or associated with P. acnes. Moreover, DPPE 1.2 associated to P. acnes was non-toxic to treated animals. The destruction of L. (L.) amazonensis by DPPE 1.2 was followed by host inflammatory responses which were exacerbated when the palladacycle complex was associated with P. acnes.en
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Farmacol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Farmacol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP)
dc.description.sponsorshipFAPESP
dc.description.sponsorshipIDFAPESP: 2013/02133-8
dc.description.sponsorshipIDFAPESP: 2009/10809-6
dc.description.sponsorshipIDFAPESP: 2014/06935-4
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2017.00333
dc.identifier.citationFrontiers In Microbiology. Lausanne, v. 8, p. -, 2017.
dc.identifier.doi10.3389/fmicb.2017.00333
dc.identifier.fileWOS000395238500001.pdf
dc.identifier.issn1664-302X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54937
dc.identifier.wosWOS:000395238500001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcutaneous leishmaniasisen
dc.subjectLeishmania (Leishmania) amazonensisen
dc.subjectpalladacycle complexen
dc.subjectadjuvanten
dc.subjectPropionibacterium acnesen
dc.titleTreatment of Leishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-Killed Propionibacterium acnes Triggers Protective Cellular Immune Responsesen
dc.typeinfo:eu-repo/semantics/article
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