Physical exercise alters the activation of downstream proteins related to BDNF-TrkB signaling in male Wistar rats with epilepsy

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dc.citation.issue5
dc.citation.volumev. 96
dc.contributor.authorAlmeida, Alexandre Aparecido de [UNIFESP]
dc.contributor.authorSilva, Sergio Gomes da
dc.contributor.authorLopim, Glauber Menezes [UNIFESP]
dc.contributor.authorCampos, Diego Vannucci [UNIFESP]
dc.contributor.authorFernandes, Jansen [UNIFESP]
dc.contributor.authorCabral, Francisco Romero
dc.contributor.authorArida, Ricardo Mario [UNIFESP]
dc.coverageHoboken
dc.date.accessioned2020-07-20T16:30:58Z
dc.date.available2020-07-20T16:30:58Z
dc.date.issued2018
dc.description.abstractThere are a considerable number of studies concerning the behavioral effects of physical exercise on the epileptic brainen
dc.description.abstracthowever, the intracellular signaling mechanisms involved remain unclear. We investigated the effects of aerobic exercise on hippocampal levels of brain-derived neurotrophic factor (BDNF), expression of its receptor tropomyosin receptor kinase B (TrkB), and activation of intracellular proteins related to BDNF-TrkB signaling in male Wistar rats with pilocarpine-induced epilepsy. Thirty days after the first spontaneous seizure, rats from the exercise group undertook a 30-day physical exercise program on the treadmill. Thereafter, BDNF levels, expression of TrkB, and activation of intracellular proteins were quantified by enzyme-linked immunosorbent assay, Western blotting, and multiplex assay, respectively. Statistical analyses were conducted using nonparametric tests. Rats with epilepsy presented decreased BDNF levels compared with control rats. BDNF levels increased significantly in the exercise group compared with the epileptic and control groups. Expression of full-length and truncated TrkB was increased in rats with epilepsy, and physical exercise restored its expression to control levels. RAC-alpha serine/threonine-protein kinase, mammalian target of rapamycin, and extracellular signal-regulated kinase activation were reduced in rats with epilepsy, and exercise increased activation compared with control and epilepsy groups. Increased cAMP response element binding protein activation was observed in the exercise group compared with the epilepsy group. Our findings indicate that the beneficial effects of exercise in the epileptic brain can be in part related to alterations in the activation of proteins related to the BDNF-TrkB signaling pathway.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Fisiol, Rua Botucatu 862,Ed Ciencias Biomed,5 Andar, BR-04023900 Sao Paulo, SP, Brazil
dc.description.affiliationnst Fed Goiano, Campus Ceres, Ceres, Brazil
dc.description.affiliationHosp Israelita Albert Einstein, Sao Paulo, Brazil
dc.description.affiliationUniv Mogi das Cruzes, Mogi Das Cruzes, Brazil
dc.description.affiliationSanta Casa Sao Paulo, Fac Ciencias Med, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Fisiol, Rua Botucatu 862,Ed Ciencias Biomed,5 Andar, BR-04023900 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo
dc.description.sponsorshipPrograma Nucleos de Excelencia (FAPESP-MCT/CNPq-PRONEX)
dc.description.sponsorshipIDCNPq: 300605/2013-07
dc.description.sponsorshipIDFAPESP: 14/00035-1
dc.description.sponsorshipIDFAPESP: 2013/12692-4
dc.description.sponsorshipIDFAPESP: 2013/13725-3
dc.description.sponsorshipIDNucleos de Excelencia: 2011/50680-2
dc.format.extent911-920
dc.identifierhttp://dx.doi.org/10.1002/jnr.24196
dc.identifier.citationJournal Of Neuroscience Research. Hoboken, v. 96, n. 5, p. 911-920, 2018.
dc.identifier.doi10.1002/jnr.24196
dc.identifier.issn0360-4012
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55618
dc.identifier.wosWOS:000427481100016
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofJournal Of Neuroscience Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBDNFen
dc.subjectcell signalingen
dc.subjectepilepsyen
dc.subjectphysical exerciseen
dc.titlePhysical exercise alters the activation of downstream proteins related to BDNF-TrkB signaling in male Wistar rats with epilepsyen
dc.typeinfo:eu-repo/semantics/article
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