Effect of Cryopreservation on Human Adipose Tissue and Isolated Stromal Vascular Fraction Cells: In Vitro and In Vivo Analyses

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dc.citation.issue2
dc.citation.volume141
dc.contributor.authorZanata, Fabiana [UNIFESP]
dc.contributor.authorBowles, Annie
dc.contributor.authorFrazier, Trivia
dc.contributor.authorCurley, J. Lowry
dc.contributor.authorBunnell, Bruce A.
dc.contributor.authorWu, Xiying
dc.contributor.authorWade, James
dc.contributor.authorDevireddy, Ram
dc.contributor.authorGimble, Jeffrey M.
dc.contributor.authorFerreira, Lydia Masako [UNIFESP]
dc.coveragePhiladelphia
dc.date.accessioned2020-07-08T13:09:45Z
dc.date.available2020-07-08T13:09:45Z
dc.date.issued2018
dc.description.abstractBackground: Adipose tissue is a source of adipose-derived stromal/stem cells for tissue engineering and reconstruction and a tissue source for fat grafts. Although liposuction is a simple procedure for the harvest of adipose tissue, the repetition of this surgical intervention can cause adverse effects to the patient and can be a limiting factor for immediate use. Cryopreservation can avoid the morbidity associated with repetitive liposuction, allowing the use of stored tissue after the initial harvest procedure. This article focuses on the characterization of fresh and cryopreserved human adipose tissue. Methods: Lipoaspirates from eight donors were processed as fresh adipose tissue or cryopreserved for 4 to 6 weeks. Fresh and cryopreserved tissues were collagenase digested and the stromal vascular fraction cells were characterized immediately or cryopreserved. Characterization was based on stromal vascular fraction cell proliferation and immunophenotype. In vivo fat grafting was performed in C57BL/6 green fluorescent protein mice to analyze morphology of the tissue and its adiposity using confocal microscopy, histochemical staining (i.e., hematoxylin and eosin and Masson trichrome), and immunohistochemistry (i.e., green fluorescent protein, perilipin, and CD31). Results: Although tissue and stromal vascular fraction cell cryopreservation reduced the total cell yield, the remaining viable cells retained their adhesive and proliferative properties. The stromal vascular fraction cell immunophenotype showed a significant reduction in the hematopoietic surface markers and increased expression of stromal and adipogenic markers following cryopreservation. In vivo cryopreserved fat grafts showed morphology similar to that of freshly implanted fat grafts. Conclusion: In this study, the authors demonstrated that cryopreserved adipose tissue is a potential source of stromal vascular fraction cells and a suitable source for fat grafts.en
dc.description.affiliationUniv Fed Sao Paulo, Translat Surg Grad Program, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Plast Surg Div, Sao Paulo, Brazil
dc.description.affiliationTulane Univ, Ctr Stem Cell Res & Regenerat Med, New Orleans, LA 70118 USA
dc.description.affiliationTulane Univ, Dept Cell & Mol Biol, Sch Sci & Engn, New Orleans, LA 70118 USA
dc.description.affiliationDillard Univ, Phys Dept, New Orleans, LA USA
dc.description.affiliationLa Cell LLC, New Orleans, LA USA
dc.description.affiliationTulane Univ, Sch Med, Dept Pharmacol, New Orleans, LA 70112 USA
dc.description.affiliationTulane Univ, Sch Med, Dept Med, New Orleans, LA 70112 USA
dc.description.affiliationTulane Univ, Sch Med, Dept Struct & Cellular Biol, 1430 Tulane Ave, New Orleans, LA 70112 USA
dc.description.affiliationTulane Univ, Sch Med, Dept Surg, New Orleans, LA 70112 USA
dc.description.affiliationTulane Natl Primate Res Ctr, Covington, LA USA
dc.description.affiliationPlast Surg Associates, Ft Collins, CO USA
dc.description.affiliationLouisiana State Univ, Dept Mech Engn, Baton Rouge, LA 70803 USA
dc.description.affiliationCoordenacao Aperfeicoamento Pessoal Nivel Super, Brasilia, DF, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Translat Surg Grad Program, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Plast Surg Div, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCAPES, Brazil
dc.description.sponsorshipLa Cell LLC
dc.description.sponsorshipIDCAPES, Brazil: BEX 1524/15-1
dc.format.extent232E-243E
dc.identifierhttp://dx.doi.org/10.1097/PRS.0000000000004030
dc.identifier.citationPlastic And Reconstructive Surgery. Philadelphia, v. 141, n. 2, p. 232E-243E, 2018.
dc.identifier.doi10.1097/PRS.0000000000004030
dc.identifier.issn0032-1052
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54184
dc.identifier.wosWOS:000425474200005
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofPlastic And Reconstructive Surgery
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.titleEffect of Cryopreservation on Human Adipose Tissue and Isolated Stromal Vascular Fraction Cells: In Vitro and In Vivo Analysesen
dc.typeinfo:eu-repo/semantics/article
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