Purification and preliminary characterization of a plasma kallikrein inhibitor isolated from sea hares Aplysia dactylomela Rang, 1828

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dc.contributor.authorGonzalez, Y.
dc.contributor.authorAraujo, M. S.
dc.contributor.authorOliva, MLV
dc.contributor.authorSampaio, CAM
dc.contributor.authorChavez, M. A.
dc.contributor.institutionUniv La Habana
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:34:17Z
dc.date.available2016-01-24T12:34:17Z
dc.date.issued2004-02-01
dc.description.abstractAn inhibitor active against pancreatic trypsin was found in the crude extract from the sea hares Aplysia dactylomela Rang, 1828. A stronger inhibitory activity against human plasma kallikrein was detectable after treating this extract at 60 T, for 30 min. the plasma kallikrein inhibitor (AdKI) purification was achieved by acetone fractionation (80%) v/v, ion-exchange chromatography on Mono Q column and gel filtration chromatography on Superdex 75 column (FPLC system). By the latter a molecular mass of 2,900 Da was estimated. the purified inhibitor strongly inhibits human plasma kallikrein with a K-i value of 2.2 X 10(-10) M, while human plasmin and pancreatic trypsin were inhibited with K-i values of 1.8 X 10(-9) and 4.7 x 10(-9) M, respectively. Chymotrypsin, pancreatic elastase, pancreatic kallikrein and thrombin are not inhibited. the effect of AdKI on plasma kallikrein was confirmed by the prolongation of activated partial thromboplastin time, using a clotting time assay. the inhibitor did not affect prothrombin time or thrombin time. AdKi is a more specific inhibitor than other serine proteinase inhibitors from marine invertebrates. (C) 2003 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv La Habana, Ctr Estudio Proteinas, Fac Biol, Bangkok 10400, Thailand
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent219-223
dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2003.11.016
dc.identifier.citationToxicon. Oxford: Pergamon-Elsevier B.V., v. 43, n. 2, p. 219-223, 2004.
dc.identifier.doi10.1016/j.toxicon.203.11.016
dc.identifier.issn0041-0101
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27613
dc.identifier.wosWOS:000220153500013
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofToxicon
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectAplysia dactylomelaen
dc.subjectmolluscaen
dc.subjectproteinase inhibitoren
dc.subjectserine proteinaseen
dc.titlePurification and preliminary characterization of a plasma kallikrein inhibitor isolated from sea hares Aplysia dactylomela Rang, 1828en
dc.typeinfo:eu-repo/semantics/article
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