Inducible nitric oxide synthase inhibition attenuates lung tissue responsiveness and remodeling in a model of chronic pulmonary inflammation in guinea pigs

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dc.contributor.authorStarling, Claudia Miranda
dc.contributor.authorPrado, Carla Máximo [UNIFESP]
dc.contributor.authorLeick-Maldonado, Edna Aparecida
dc.contributor.authorLancas, Tatiana
dc.contributor.authorReis, Fabiana Gomes
dc.contributor.authorAristoteles, Luciana Ritha de Cassia Rolim Barbosa
dc.contributor.authorDolhnikoff, Marisa
dc.contributor.authorMartins, Milton de Arruda
dc.contributor.authorTiberio, Iolanda de Fátima Lopes Calvo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:52:17Z
dc.date.available2016-01-24T13:52:17Z
dc.date.issued2009-02-28
dc.description.abstractWe evaluated the influence of iNOS-derived NO on the mechanics, inflammatory, and remodeling process in peripheral lung parenchyma of guinea pigs with chronic pulmonary allergic inflammation. Animals treated or not with 1400W were submitted to seven exposures of ovalbumin in increasing doses. Seventy-two hours after the 7th inhalation, lung strips were suspended in a Krebs organ bath, and tissue resistance and elastance measured at baseline and after ovalbumin challenge. the strips were submitted to histopathological measurements. the ovalbumin-exposed animals showed increased maximal responses of resistance and elastance (p < 0.05), eosinophils counting (p < 0.001), iNOS-positive cells (p < 0.001), collagen and elastic fiber deposition (p < 0.05), actin density (p < 0.05) and 8-iso-PGF2 alpha expression (p < 0.001) in alveolar septa compared to saline-exposed ones. Ovalbumin-exposed animals treated with 1400 W had a significant reduction in lung functional and histopathological findings (p < 0.05). We showed that iNOS-specific inhibition attenuates lung parenchyma constriction, inflammation, and remodeling, suggesting NO-participation in the modulation of the oxidative stress pathway. (C) 2008 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, Dept Med, Sch Med, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Dept Pathol, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biol Sci, Diadema, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biol Sci, Diadema, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipLaboratorio de Investigacao Medica do Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo (LIM 20)
dc.format.extent185-194
dc.identifierhttps://dx.doi.org/10.1016/j.resp.2008.11.011
dc.identifier.citationRespiratory Physiology & Neurobiology. Amsterdam: Elsevier B.V., v. 165, n. 2-3, p. 185-194, 2009.
dc.identifier.doi10.1016/j.resp.2008.11.011
dc.identifier.issn1569-9048
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/31328
dc.identifier.wosWOS:000264579900011
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofRespiratory Physiology & Neurobiology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectiNOSen
dc.subjectExperimental model of asthmaen
dc.subjectLung tissue constrictionen
dc.subjectCollagen depositionen
dc.titleInducible nitric oxide synthase inhibition attenuates lung tissue responsiveness and remodeling in a model of chronic pulmonary inflammation in guinea pigsen
dc.typeinfo:eu-repo/semantics/article
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