Characterization of Novel O-Glycans Isolated from Tear and Saliva of Ocular Rosacea Patients

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dc.contributor.authorOzcan, Sureyya
dc.contributor.authorAn, Hyun Joo
dc.contributor.authorVieira, Ana C. [UNIFESP]
dc.contributor.authorPark, Gun Wook
dc.contributor.authorKim, Jae Han
dc.contributor.authorMannis, Mark J.
dc.contributor.authorLebrilla, Carlito B.
dc.contributor.institutionUniv Calif Davis
dc.contributor.institutionChungnam Natl Univ
dc.contributor.institutionUniv Calif
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:31:21Z
dc.date.available2016-01-24T14:31:21Z
dc.date.issued2013-03-01
dc.description.abstractO-Glycans in saliva and tear isolated from patients suffering from ocular rosacea, a form of inflammatory ocular surface disease, were profiled, and their structures were elucidated using high resolution mass spectrometry. We have previously shown that certain structures, particularly sulfated oligosaccharides, increased in the tear and saliva of rosacea patients in this study, the structures of these glycans were elucidated using primarily tandem mass spectrometry. There were important similarities in the glycan profiles of tears and saliva with the majority of the structures in common. the structures of the most abundant species common to both tear and saliva, which were also the most abundant species in both, were elucidated. for sulfated species, the positions of the sulfate groups were localized. the majority of the structures were new, with the sulfated glycans comprising mucin core 1- and core 2-type structures. As both saliva and tear are rich in mucins, it is suggested that the O-glycans are mainly components of mucins. the study further illustrates the strong correspondence between the glycans in the tear and saliva of ocular rosacea patients.en
dc.description.affiliationUniv Calif Davis, Dept Chem, Davis, CA 95616 USA
dc.description.affiliationUniv Calif Davis, Dept Biochem & Mol Med, Davis, CA 95616 USA
dc.description.affiliationChungnam Natl Univ, Grad Sch Analyt Sci & Technol, Taejon 305764, South Korea
dc.description.affiliationChungnam Natl Univ, Dept Food Nutr, Taejon 305764, South Korea
dc.description.affiliationUniv Calif, Davis Hlth Syst Eye Ctr, Dept Ophthalmol & Vis Sci, Sacramento, CA 95817 USA
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ophthalmol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipNational Institutes of Health
dc.description.sponsorshipConverging Research Center Program through the Ministry of Education, Science and Technology
dc.description.sponsorshipIDNational Institutes of Health: RO1GM049077
dc.description.sponsorshipIDConverging Research Center Program through the Ministry of Education, Science and Technology: 2011K000968
dc.format.extent1090-1100
dc.identifierhttp://dx.doi.org/10.1021/pr3008013
dc.identifier.citationJournal of Proteome Research. Washington: Amer Chemical Soc, v. 12, n. 3, p. 1090-1100, 2013.
dc.identifier.doi10.1021/pr3008013
dc.identifier.issn1535-3893
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36043
dc.identifier.wosWOS:000315708000003
dc.language.isoeng
dc.publisherAmer Chemical Soc
dc.relation.ispartofJournal of Proteome Research
dc.rightsAcesso restrito
dc.subjectO-glycanen
dc.subjectsalivaen
dc.subjecttearen
dc.subjectmass spectrometryen
dc.subjectstructural characterizationen
dc.subjectsulfated O-glycanen
dc.subjectsialylated O-glycanen
dc.titleCharacterization of Novel O-Glycans Isolated from Tear and Saliva of Ocular Rosacea Patientsen
dc.typeArtigo
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