Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection

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dc.contributor.authorWatanabe, Aripuanã Sakurada Aranha [UNIFESP]
dc.contributor.authorCarraro, Emerson [UNIFESP]
dc.contributor.authorCamargo, Clarice Neves [UNIFESP]
dc.contributor.authorPuerari, Diane
dc.contributor.authorGuatura, Sandra
dc.contributor.authorGranato, Celso Francisco Hernandes [UNIFESP]
dc.contributor.authorBellei, Nancy Cristina Junqueira [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade do Centro-Oeste Departamento de farmacologia Clínica
dc.date.accessioned2015-06-14T13:45:24Z
dc.date.available2015-06-14T13:45:24Z
dc.date.issued2013-04-09
dc.description.abstractINTRODUCTION: Human adenoviruses (HAdV) play an important role in the etiology of severe acute lower respiratory infection, especially in immunocompromised individuals. The aim of the present study was detect the HAdV through different methods: direct fluorescence assay (DFA) and nested-polymerase chain reaction (PCR-nested) from patients with acute respiratory infection (ARI) up to 7 days of symptoms onset. METHODS: Samples (n=643) were collected from different risk groups during from 2001 to 2010: 139 adults attended in an Emergency Room Patients (ERP); 205 health care workers (HCW); 69 from Renal Transplant Outpatients (RTO); 230 patients in hematopoietic stem cell transplantation (HSCT) program. RESULTS: Among all patients (n=643) adenovirus was detected on 13.2% by DFA and/or PCR: 6/139 (4.3%) adults from ERP, 7/205 (3.4%) from HCW samples, 4/69 (5.8%) from RTO and 68/230 (29.5%) from HSCT patients. Nested PCR showed higher detection (10%) compared to DFA test (3.8%) (p < 0.001). HSCT patients presented significantly higher prevalence of HAdV infection. CONCLUSIONS: Adenovirus detection through nested-PCR assay was higher. However the inclusion of molecular method in laboratorial routine diagnostic should be evaluated considering the reality of each specific health service.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departamento de Medicina Disciplina de Infectologia
dc.description.affiliationUniversidade do Centro-Oeste Departamento de farmacologia Clínica
dc.description.affiliationUnifespUNIFESP, Depto. de Medicina Disciplina de Infectologia
dc.description.sourceSciELO
dc.format.extent161-165
dc.identifierhttp://dx.doi.org/10.1590/0037-8682-1699-2013
dc.identifier.citationRevista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 2, p. 161-165, 2013.
dc.identifier.doi10.1590/0037-8682-1699-2013
dc.identifier.fileS0037-86822013000200161.pdf
dc.identifier.issn0037-8682
dc.identifier.scieloS0037-86822013000200161
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/7732
dc.language.isoeng
dc.publisherSociedade Brasileira de Medicina Tropical - SBMT
dc.relation.ispartofRevista da Sociedade Brasileira de Medicina Tropical
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHAdVen
dc.subjectRespiratory infectionen
dc.subjectDFAen
dc.subjectNested PCRen
dc.subjectImmunocompromised patients and immunocompetent patientsen
dc.titleHuman adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infectionen
dc.typeinfo:eu-repo/semantics/article
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