Galactosaminoglycans from normal myometrium and leiomyoma

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dc.contributor.authorBerto, A.g.a. [UNIFESP]
dc.contributor.authorOba-Shinjo, Sueli Mieko [UNIFESP]
dc.contributor.authorMichelacci, Yara Maria [UNIFESP]
dc.contributor.authorSampaio, L.o. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:29:23Z
dc.date.available2015-06-14T13:29:23Z
dc.date.issued2001-05-01
dc.description.abstractIn many tumors, the amount of chondroitin sulfate in the extracellular matrix has been shown to be elevated when compared to the corresponding normal tissue. Nevertheless, the degree of chondroitin sulfate increase varies widely. In order to investigate a possible correlation between the amount of chondroitin sulfate and tumor size, several individual specimens of human leiomyoma, a benign uterine tumor, were analyzed. The glycosaminoglycans from eight tumors were extracted and compared with those from the respective adjacent normal myometrium. The main glycosaminoglycan found in normal myometrium was dermatan sulfate, with small amounts of chondroitin sulfate and heparan sulfate. In leiomyoma, both dermatan sulfate and chondroitin sulfate were detected and the total amounts of the two galactosaminoglycans was increased in all tumors when compared to normal tissue. In contrast, the heparan sulfate concentration decreased in the tumor. To assess the disaccharide composition of galactosaminoglycans, these compounds were incubated with bacterial chondroitinases AC and ABC. The amounts of L-iduronic acid-containing disaccharides remained constant, whereas the concentration of D-glucuronic acid-containing disaccharides increased from 2 to 10 times in the tumor, indicating that D-glucuronic acid-containing disaccharides are responsible for the elevation in galactosaminoglycan concentration. This increase is positively correlated with tumor size.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Bioquímica
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Bioquímica
dc.description.sourceSciELO
dc.format.extent633-637
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2001000500011
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 5, p. 633-637, 2001.
dc.identifier.doi10.1590/S0100-879X2001000500011
dc.identifier.fileS0100-879X2001000500011.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2001000500011
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/1155
dc.identifier.wosWOS:000168735900011
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectgalactosaminoglycanen
dc.subjectglycosaminoglycanen
dc.subjectchondroitin sulfateen
dc.subjectdermatan sulfateen
dc.subjectleiomyomaen
dc.subjectmyometriumen
dc.titleGalactosaminoglycans from normal myometrium and leiomyomaen
dc.typeinfo:eu-repo/semantics/article
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