Morphological events during the Trypanosoma cruzi cell cycle

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dc.contributor.authorElias, Maria Carolina
dc.contributor.authorCunha, Julia P. C. da
dc.contributor.authorFaria, Flavio P. de
dc.contributor.authorMortara, Renato A.
dc.contributor.authorFreymueller, Edna
dc.contributor.authorSchenkman, Sergio
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInst Butantan
dc.date.accessioned2016-01-24T12:42:02Z
dc.date.available2016-01-24T12:42:02Z
dc.date.issued2007-04-01
dc.description.abstractThe replication and segregation of organelles producing two identical daughter cells must be precisely controlled during the cell cycle progression of eukaryotes. in kinetoplastid flagellated protozoa, this includes the duplication of the single mitochondrion containing a network of DNA, known as the kinetoplast, and a flagellum that grows from a cytoplasmic basal body through the flagellar pocket compartment before emerging from the cell. Here, we show the morphological events and the timing of these events during the cell cycle of the epimastigote form of Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease. DNA staining, flagellum labeling, bromodeoxyuridine incorporation, and ultra-thin serial sections show that nuclear replication takes 10% of the whole cell cycle time. in the middle of the G2 stage, the new flagellum emerges from the flagellar pocket and grows unattached to the cell body. While the new flagellum is still short, the kinetoplast segregates and mitosis occurs. the new flagellum reaches its final size during cytokinesis when a new cell body is formed. These precisely coordinated cell cycle events conserve the epimastigote morphology with a single nucleus, a single kinetoplast, and a single flagellum status of the interphasic cell. (C) 2006 Elsevier GmbH. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationInst Butantan, Parasitol Lab, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Ctr Microscopia Eletron, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Ctr Microscopia Eletron, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent147-157
dc.identifierhttp://dx.doi.org/10.1016/j.protis.2006.10.002
dc.identifier.citationProtist. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 158, n. 2, p. 147-157, 2007.
dc.identifier.doi10.1016/j.protis.2006.10.002
dc.identifier.issn1434-4610
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29634
dc.identifier.wosWOS:000246105100003
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofProtist
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectcell cycleen
dc.subjectflagellumen
dc.subjectkinetoplasten
dc.subjectnucleusen
dc.subjectmitosisen
dc.subjectTypanosoma cruzien
dc.titleMorphological events during the Trypanosoma cruzi cell cycleen
dc.typeinfo:eu-repo/semantics/article
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