Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices

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dc.citation.issue3
dc.citation.volume47
dc.contributor.authorDellagnezze, Bruna Martins
dc.contributor.authorde Vasconcellos, Suzan Pantaroto
dc.contributor.authorde Melo, Itamar Soares
dc.contributor.authordos Santos Neto, Eugenio Vaz
dc.contributor.authorde Oliveira, Valeria Maia
dc.coverageSao Paulo
dc.date.accessioned2020-08-14T13:44:24Z
dc.date.available2020-08-14T13:44:24Z
dc.date.issued2016
dc.description.abstractUnraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. Despite the techniques developed in order to improve methodologies involving DNA extraction from crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from environments with low cellular density for further microbiological analyses. This work aimed at the evaluation of different matrices (arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization was carried out using Scanning Electronic Microscopy (SEM), Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA gene libraries in order to compare the microbial biomass yield, DNA recovery efficiency and diversity among the enrichments. The DNA from microbial communities in petroleum enrichments was purified according to a protocol established in this work and used for 16S rRNA amplification with bacterial generic primers. The PCR products were cloned, and positive clones were screened by Amplified Ribosomal DNA Restriction Analysis (ARDRA). Sequencing and phylogenetic analyses revealed that the bacterial community was mostly represented by members of the genera Petrotoga, Bacillus, Pseudomonas, Geobacillus and Rahnella. The use of different support materials in the enrichments yielded an increase in microbial biomass and biofilm formation, indicating that these materials may be employed for efficient biomass recovery from petroleum reservoir samples. Nonetheless, the most diverse microbiota were recovered from the biodegraded petroleum sample using polyurethane foam cubes as support material. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. This is an open access article under the CC BY-NC-ND licenseen
dc.description.affiliationUniv Campinas UNICAMP, Res Ctr Chem, Div Microbial Resources, Biol,Agr CPQBA, Campinas, SP, Brazil
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Dept Biol Sci, Diadema, SP, Brazil
dc.description.affiliationEMBRAPA, Natl Ctr Res Monitoring & Evaluating Environm Imp, Jaguariuna, SP, Brazil
dc.description.affiliationPETROBRAS R&D Ctr, Rio De Janeiro, RJ, Brazil
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Dept Biol Sci, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent712-723
dc.identifierhttp://dx.doi.org/10.1016/j.bjm.2016.04.004
dc.identifier.citationBrazilian Journal Of Microbiology. Sao Paulo, v. 47, n. 3, p. 712-723, 2016.
dc.identifier.doi10.1016/j.bjm.2016.04.004
dc.identifier.fileWOS000381101200026.pdf
dc.identifier.issn1517-8382
dc.identifier.scieloS1517-83822016000300712
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57647
dc.identifier.wosWOS:000381101200026
dc.language.isoeng
dc.publisherSoc Brasileira Microbiologia
dc.relation.ispartofBrazilian Journal Of Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBacterial diversityen
dc.subjectMicrobial enrichmenten
dc.subjectPetroleum reservoiren
dc.subjectPhysical supporten
dc.subject16S rRNAen
dc.titleEvaluation of bacterial diversity recovered from petroleum samples using different physical matricesen
dc.typeinfo:eu-repo/semantics/article
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