Ultrastructural morphometric analysis of ameloblasts exposed to fluoride during tooth development

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dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorHirota, Luciane
dc.contributor.authorCestari, Tania Mary
dc.contributor.authorCeolin, Daniele Santi
dc.contributor.authorTaga, Rumio
dc.contributor.authorAssis, Gerson Francisco de
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T12:41:33Z
dc.date.available2016-01-24T12:41:33Z
dc.date.issued2006-11-01
dc.description.abstractSince a considerable amount of the world population is exposed to high doses of fluoride, it is of special concern to investigate its action mechanisms during dental enamel development. in this study, the toxicity of fluoride in ameloblasts during enamel development was evaluated by means of ultrastructural morphometric analysis. A total of 18 male Wistar rats were distributed into three groups. in Group I, the animals received deionized drinking water ad libitum (negative control) and in Groups II and III, they received sodium fluorided (NaF) drinking water at doses of 7 and 100 ppm ad libitum, respectively, for 6 weeks. Morphometric data were expressed as volume density of the most significant organelles present in the secretory and maturation phases of amelogenesis such as RER, granules, lysosomes, phagic vacuoles, microfilaments and mitochondria. the results showed that the volume density of mitochondria in the 100 ppm experimental group was 29% (P < 0.05) higher than the control group in secretory ameloblasts. No remarkable differences were found in maturation ameloblasts for all organelles evaluated. Taken together, these data indicate that NaF at high doses is able to induce cellular damage in secretory ameloblasts, whereas no noxious effect was observed during maturation stage of amelogenesis as depicted by ultrastructural analysis.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Hlth Sci, BR-11060100 Santos, SP, Brazil
dc.description.affiliationUniv São Paulo, Bauru SCh Dent, Dept Biol Sci, Histol Lab, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Hlth Sci, BR-11060100 Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent361-367
dc.identifierhttps://dx.doi.org/10.1007/s10735-006-9065-2
dc.identifier.citationJournal of Molecular Histology. Dordrecht: Springer, v. 37, n. 8-9, p. 361-367, 2006.
dc.identifier.doi10.1007/s10735-006-9065-2
dc.identifier.issn1567-2379
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/29225
dc.identifier.wosWOS:000242435700006
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofJournal of Molecular Histology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectDental fluorosisen
dc.subjectFluorideen
dc.subjectAmeloblasten
dc.subjectRat incisoren
dc.subjectUltrastructureen
dc.titleUltrastructural morphometric analysis of ameloblasts exposed to fluoride during tooth developmenten
dc.typeinfo:eu-repo/semantics/article
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