Proteomic study revealed cellular assembly and lipid metabolism dysregulation in sepsis secondary to community-acquired pneumonia

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dc.citation.volume7
dc.contributor.authorSharma, Narendra Kumar [UNIFESP]
dc.contributor.authorTashima, Alexandre Keiji [UNIFESP]
dc.contributor.authorColo Brunialti, Milena Karina [UNIFESP]
dc.contributor.authorFerreira, Eden Ramalho [UNIFESP]
dc.contributor.authorSoares Torquato, Ricardo Jose [UNIFESP]
dc.contributor.authorMortara, Renato Arruda [UNIFESP]
dc.contributor.authorMachado, Flavia Ribeiro [UNIFESP]
dc.contributor.authorAssuncao, Murillo
dc.contributor.authorRigato, Otelo [UNIFESP]
dc.contributor.authorSalomao, Reinaldo [UNIFESP]
dc.coverageLondon
dc.date.accessioned2020-09-01T13:21:17Z
dc.date.available2020-09-01T13:21:17Z
dc.date.issued2017
dc.description.abstractSepsis is a life-threatening disorder characterized by organ dysfunction and a major cause of mortality worldwide. The major challenge in studying sepsis is its diversity in such factors as age, source of infection and etiology. Recently, genomic and proteomic approaches have improved our understanding of its complex pathogenesis. In the present study, we use quantitative proteomics to evaluate the host proteome response in septic patients secondary to community-acquired pneumonia (CAP). Samples obtained at admission and after 7 days of follow-up were analyzed according to the outcomes of septic patients. The patients' proteome profiles were compared with age-and gender-matched healthy volunteers. Bioinformatic analyses of differentially expressed proteins showed alteration in the cytoskeleton, cellular assembly, movement, lipid metabolism and immune responses in septic patients. Actin and gelsolin changes were assessed in mononuclear cells using immunofluorescence, and a higher expression of gelsolin and depletion of actin were observed in survivor patients. Regarding lipid metabolism, changes in cholesterol, HDL and apolipoproteins were confirmed using enzymatic colorimetric methods in plasma. Transcriptomic studies revealed a massive change in gene expression in sepsis. Our proteomic results stressed important changes in cellular structure and metabolism, which are possible targets for future interventions of sepsis.en
dc.description.affiliationUniv Fed Sao Paulo, Hosp Sao Paulo, Div Infect Dis, Escola Paulista Med, BR-04039032 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biochem, Escola Paulista Med, BR-04023900 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Escola Paulista Med, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Intens Care Unit, Hosp Sao Paulo, Escola Paulista Med, BR-04024002 Sao Paulo, Brazil
dc.description.affiliationHosp Israelita Albert Einstein, Intens Care Unit, BR-05652900 Sao Paulo, Brazil
dc.description.affiliationHosp Sirio Libanes, Intens Care Unit, BR-01409001 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Hosp Sao Paulo, Div Infect Dis, Escola Paulista Med, BR-04039032 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biochem, Escola Paulista Med, BR-04023900 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Escola Paulista Med, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Intens Care Unit, Hosp Sao Paulo, Escola Paulista Med, BR-04024002 Sao Paulo, Brazil
dc.description.provenanceMade available in DSpace on 2020-09-01T13:21:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2017. Added 1 bitstream(s) on 2020-09-02T13:06:31Z : No. of bitstreams: 1 WOS000415265800017.pdf: 4788359 bytes, checksum: 8a22954b76e9e0b4749b5ddf32f64134 (MD5)en
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico, CNPq
dc.description.sponsorshipFAPESP
dc.description.sponsorshipIDFAPESP: 2011/20401-4
dc.description.sponsorshipIDFAPESP: 2013/15636-8
dc.description.sponsorshipIDCNPq: 305685/2011-2
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1038/s41598-017-15755-1
dc.identifier.citationScientific Reports. London, v. 7, p. -, 2017.
dc.identifier.doi10.1038/s41598-017-15755-1
dc.identifier.fileWOS000415265800017.pdf
dc.identifier.issn2045-2322
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/58170
dc.identifier.wosWOS:000415265800017
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofScientific Reports
dc.rightsAcesso aberto
dc.titleProteomic study revealed cellular assembly and lipid metabolism dysregulation in sepsis secondary to community-acquired pneumoniaen
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