Effects of estrogen on intracellular signaling pathways linked to activation of muscarinic acetylcholine receptors and on acetylcholinesterase activity in rat hippocampus

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dc.contributor.authorSantos Pereira, Renato Tavares dos
dc.contributor.authorPorto, Catarina Segreti [UNIFESP]
dc.contributor.authorGodinho, Rosely Oliveira [UNIFESP]
dc.contributor.authorFrancis Abdalla, Fernando Mauricio
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:49:47Z
dc.date.available2016-01-24T13:49:47Z
dc.date.issued2008-05-01
dc.description.abstractThe aim of the present study was to investigate the effects of estrogen lack and estrogen replacement on the production of total [H-3]inositol phosphate ([H-3]IP) induced by the activation of muscarinic acetylcholine receptors (mAChRs) and on the mechanisms for inactivation of acetylcholine. Hippocampi were obtained from rats in proestrus (PE), ovariectomized for 15 days (C15), ovariectomized for 15 days and then treated with 17 beta-estradiol for 7 days (E7) and ovariectomized and immediately treated with 17 beta-estradiol for 21 days (E21). Ovariectomy did not change the basal level of total [H-3]IP in the hippocampus. 17 beta-Estradiol replacement (E7 and E21) reduced the basal level of total [H-3]IP. in all experimental groups, carbachol (CCh) caused a concentration -dependent rise in total [H-3]IP. the maximum effect was reached with 10(-4) M CCh. the response to 10(-4) M CCh in the hippocampi from C15 and E7 rats was twofold higher than in hippocampi from PE and E21 animals and was blocked by pirenzepine, but not by methoctramine. Ovariectomy or 17 beta-estradiol treatment for 7 days did not change neither the total acetylcholinesterase (AChE) activity nor the relative amount of mono- and dimeric G(1)/G(2) and tetrameric G(4) globular forms. Conversely, hormonal treatment for 21 days induced an increase in AChE activity of G(1)/G(2) and G(4) forms, indicating that 17 beta-estradiol stimulates both synthesis and assembly of AChE molecular forms. the present results suggest that the duration and/or a critical period with regard to the initiation of estrogen therapy are important to regulate the function of mAChRs and AChE activity in female rat hippocampus. (C) 2008 Elsevier Inc. All rights reserved.en
dc.description.affiliationInst Butantan, Pharmacol Lab, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, Escola Paulista Med, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pharmacol, Div Cellular Pharmacol, Escola Paulista Med, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, Escola Paulista Med, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pharmacol, Div Cellular Pharmacol, Escola Paulista Med, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1827-1834
dc.identifierhttp://dx.doi.org/10.1016/j.bcp.2008.01.016
dc.identifier.citationBiochemical Pharmacology. Oxford: Pergamon-Elsevier B.V., v. 75, n. 9, p. 1827-1834, 2008.
dc.identifier.doi10.1016/j.bcp.2008.01.016
dc.identifier.issn0006-2952
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30635
dc.identifier.wosWOS:000255849400014
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochemical Pharmacology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectmuscarinic acetylcholine receptorsen
dc.subjecthippocampusen
dc.subjectestrogenen
dc.subjectovariectomyen
dc.subjectinositol phosphateen
dc.subjectacetylcholinesteraseen
dc.titleEffects of estrogen on intracellular signaling pathways linked to activation of muscarinic acetylcholine receptors and on acetylcholinesterase activity in rat hippocampusen
dc.typeinfo:eu-repo/semantics/article
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