Long-Term Effects of Anterior Thalamic Nucleus Deep Brain Stimulation on Spatial Learning in the Pilocarpine Model of Temporal Lobe Epilepsy

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dc.citation.issue2
dc.citation.volume21
dc.contributor.authorFerreira, Elenn Soares [UNIFESP]
dc.contributor.authorVieira, Lais Gabrielle [UNIFESP]
dc.contributor.authorMoraes, Daniela Macedo [UNIFESP]
dc.contributor.authorAmorim, Beatriz O. [UNIFESP]
dc.contributor.authorMalheiros, Jackeline Moraes [UNIFESP]
dc.contributor.authorHamani, Clement
dc.contributor.authorCovolan, Luciene [UNIFESP]
dc.coverageHoboken
dc.date.accessioned2020-07-08T13:09:48Z
dc.date.available2020-07-08T13:09:48Z
dc.date.issued2018
dc.description.abstractIntroduction and ObjectivesCognitive impairment is a significant comorbidity of temporal lobe epilepsy that is associated with extensive hippocampal cell loss. Deep brain stimulation (DBS) of the anterior thalamic nucleus (ANT) has been used for the treatment of refractory partial seizures. In the pilocarpine model of epilepsy, ANT DBS applied during status epilepticus (SE) reduces hippocampal inflammation and apoptosis. When given to chronic epileptic animals it reduces hippocampal excitability and seizure frequency. Here, we tested whether ANT DBS delivered during SE and the silent phase of the pilocarpine model would reduce cognitive impairment when animals became chronically epileptic. Materials and MethodsSE was induced by a systemic pilocarpine injection (320 mg/kg). Immediately after SE onset, rats were assigned to receive DBS during the first six hours of SE (n=8en
dc.description.abstractDBSa group) or during SE+the silent period (i.e., 6 h/day until the animals developed the first spontaneous recurrent seizureen
dc.description.abstractn=10en
dc.description.abstractDBSs group). Four months following SE, animals underwent water maze testing and histological evaluation. Nonstimulated chronic epileptic animals (n=13en
dc.description.abstractPCTL group) and age-matched naive rats (n=11, CTL group) were used as controls. Results were analyzed by repeated-measures analyses of variance (RM_ANOVA) and one-way ANOVAs, followed by Newman-Keuls post hoc tests. ResultsAlthough all groups learned the spatial task, epileptic animals with or without DBS spent significantly less time in the platform quadrant, denoting a spatial memory deficit (p<0.02). Despite these negative behavioral results, we found that animals given DBS had a significantly higher number of cells in the CA1 region and dentate gyrus. Mossy fiber sprouting was similar among all epileptic groups. ConclusionsDespite lesser hippocampal neuronal loss, ANT DBS delivered either during SE or during SE and the silent phase of the pilocarpine model did not mitigate memory deficits in chronic epileptic rats.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Fisiol, Rua Botucatu 862, BR-04023062 Sao Paulo, SP, Brazil
dc.description.affiliationCtr Addict & Mental Hlth, Behav Neurobiol Lab, Toronto, ON, Canada
dc.description.affiliationUniv Toronto, Toronto Western Hosp, Div Neurosurg, Toronto, ON, Canada
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Fisiol, Rua Botucatu 862, BR-04023062 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP
dc.description.sponsorshipCNPq
dc.description.sponsorshipCAPES
dc.description.sponsorshipIDFAPESP [2012/50950-2]
dc.description.sponsorshipIDCNPq [304021/2015-6]
dc.description.sponsorshipIDCAPES
dc.format.extent160-167
dc.identifierhttp://dx.doi.org/10.1111/ner.12688
dc.identifier.citationNeuromodulation. Hoboken, v. 21, n. 2, p. 160-167, 2018.
dc.identifier.doi10.1111/ner.12688
dc.identifier.issn1094-7159
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54214
dc.identifier.wosWOS:000424282700006
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofNeuromodulation
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectdeep brain stimulationen
dc.subjecthippocampusen
dc.subjectlearningen
dc.subjectneuroprotectionen
dc.subjectpilocarpineen
dc.subjectthalamusen
dc.subjectwater mazeen
dc.titleLong-Term Effects of Anterior Thalamic Nucleus Deep Brain Stimulation on Spatial Learning in the Pilocarpine Model of Temporal Lobe Epilepsyen
dc.typeinfo:eu-repo/semantics/article
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