Urinary excretion of glycosaminoglycans and albumin in experimental diabetes mellitus

Página do item simplificado
dc.contributor.authorCadaval, Augusto de Miranda [UNIFESP]
dc.contributor.authorKohlman, Oswaldo [UNIFESP]
dc.contributor.authorMichelacci, Yara Maria [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:31:00Z
dc.date.available2016-01-24T12:31:00Z
dc.date.issued2000-02-01
dc.description.abstractDiabetes mellitus was induced in one group of rats by a single injection of streptozotocin. the glycemia, the body weight, and the blood systolic pressure were measured every week, and the 24 h urine volume and urinary excretions of creatinine, albumin and glycosaminoglycans were measured every 2 weeks, At the end of the experiment (12 weeks) the weight and the glycosaminoglycan composition of the kidneys were determined. All the diabetic animals were hyperglycemic, hypertense, and did not gain weight during all the experimental period. Albuminuria appeared from the second week on. Rat urine was shown to contain heparan sulfate, chondroitin sulfate, and dermatan sulfate, and the glycosaminoglycan excretion decreased in all diabetic animals. the onset of the change in glycosaminoglycan excretion rate was a very early event, appearing in the second week after diabetes induction. the main glycosaminoglycan found in normal rat kidney was heparan sulfate and, in contrast to the urine, the total kidney glycosaminoglycans increased in diabetic kidney, due to chondroitin sulfate and dermatan sulfate accumulation. the heparan sulfate concentration (per tissue dry weight) did not change. Our results suggest that quantification of urinary glycosaminoglycans may be a useful tool for the early diagnosis of diabetic nephropathy.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Biol Mol, Dept Bioquim, BR-04023020 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Med, Disciplina Nefrol, BR-04023020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Biol Mol, Dept Bioquim, BR-04023020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Med, Disciplina Nefrol, BR-04023020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent185-192
dc.identifierhttp://dx.doi.org/10.1093/glycob/10.2.185
dc.identifier.citationGlycobiology. Oxford: Oxford Univ Press, v. 10, n. 2, p. 185-192, 2000.
dc.identifier.doi10.1093/glycob/10.2.185
dc.identifier.issn0959-6658
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26239
dc.identifier.wosWOS:000085143700008
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofGlycobiology
dc.rightsAcesso aberto
dc.rights.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.subjectalbuminuriaen
dc.subjectdiabetes mellitusen
dc.subjectglycosaminoglycanen
dc.subjectkidneyen
dc.subjecturineen
dc.titleUrinary excretion of glycosaminoglycans and albumin in experimental diabetes mellitusen
dc.typeArtigo
Arquivos
Coleções
EPM - Artigos