Immunoexpression of aromatase and estrogen receptors beta in stem spermatogonia of bullfrogs indicates a role of estrogen in the seasonal spermatogonial mitotic activity

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dc.contributor.authorCaneguim, Breno Henrique [UNIFESP]
dc.contributor.authorLuz, Juliana Silva da
dc.contributor.authorValentini, Sandro Roberto
dc.contributor.authorCerri, Paulo Sergio [UNIFESP]
dc.contributor.authorCerri, Estela Sasso [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Estadual Paulista
dc.date.accessioned2016-01-24T14:31:09Z
dc.date.available2016-01-24T14:31:09Z
dc.date.issued2013-02-01
dc.description.abstractBullfrog stem spermatogonia, also named primordial germ cells (PGCs), show strong testosterone immunolabeling in winter, but no or weak testosterone immunoexpression in summer. Thus, the role of testosterone in these cells needs to be clarified. in this study, we proposed to evaluate whether PGCs express aromatase and estrogen receptors, and verify a possible role of estrogen in PGCs seasonal proliferation. Testes of male adult bullfrogs, collected in winter (WG) and summer (SG), were fixed and embedded in historesin, for quantitative analysis, or paraffin for immunohistochemistry (IHC). the number of haematoxylin/eosin stained PGCs/lobular area was obtained. Proliferating cell nuclear antigen (PCNA), aromatase, estrogen receptor beta (ER beta) and PCNA/ER beta double immunolabeling were detected by IHC. the number of PCNA-positive PGCs and the histological score (HSCORE) of aromatase and ER beta immunolabeled PGCs were obtained. Although the number of PGCs increased significantly in WG, a high number of PCNA-positive PGCs was observed in summer. Moreover, aromatase and ER beta HSCORE was higher in SG than WG. the results indicate that PGCs express a seasonal proliferative activity; the low mitotic activity in winter is related to the maximal limit of germ cells which can be supported in the large lobules. in SG, the increased ER beta and aromatase HSCORE suggests that testosterone is converted into estrogen from winter to summer. Moreover, the parallelism between the high PGCs mitotic activity and ER beta immunoexpression suggest a participation of estrogen in the control of the PGCs seasonal proliferative activity which guarantee the formation of new germ cysts from summer to next autumn. (c) 2012 Elsevier Inc. All rights reserved.en
dc.description.affiliationFed Univ São Paulo UNIFESP, Dept Morphol & Genet, São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Sch Pharmaceut Sci, Dept Biol Sci, Araraquara, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Sch Dent, Dept Morphol,Lab Histol & Embryol, Araraquara, Brazil
dc.description.affiliationUnifespFed Univ São Paulo UNIFESP, Dept Morphol & Genet, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.description.sponsorshipIDFAPESP: 2009/17895-5
dc.description.sponsorshipIDFUNDUNESP: 00661/04-DFP
dc.format.extent65-72
dc.identifierhttp://dx.doi.org/10.1016/j.ygcen.2012.12.002
dc.identifier.citationGeneral and Comparative Endocrinology. San Diego: Academic Press Inc Elsevier Science, v. 182, p. 65-72, 2013.
dc.identifier.doi10.1016/j.ygcen.2012.12.002
dc.identifier.fileWOS000315013100008.pdf
dc.identifier.issn0016-6480
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35893
dc.identifier.wosWOS:000315013100008
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofGeneral and Comparative Endocrinology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectAnuraen
dc.subjectTestesen
dc.subjectTestosteroneen
dc.subjectEstrogenen
dc.subjectImmunohistochemistryen
dc.subjectProliferating cell nuclear antigenen
dc.titleImmunoexpression of aromatase and estrogen receptors beta in stem spermatogonia of bullfrogs indicates a role of estrogen in the seasonal spermatogonial mitotic activityen
dc.typeinfo:eu-repo/semantics/article
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