Distinct effects of acute and chronic sleep loss on DNA damage in rats

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dc.contributor.authorAndersen, Monica Levy [UNIFESP]
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorBergamaschi, Cassia Toledo [UNIFESP]
dc.contributor.authorAlvarenga, Tathiana Aparecida [UNIFESP]
dc.contributor.authorSilva, Andressa [UNIFESP]
dc.contributor.authorZager, Adriano [UNIFESP]
dc.contributor.authorCampos, Ruy Ribeiro [UNIFESP]
dc.contributor.authorTufik, Sergio [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:52:27Z
dc.date.available2016-01-24T13:52:27Z
dc.date.issued2009-04-30
dc.description.abstractThe aim of this investigation was to evaluate genetic damage induced in male rats by experimental sleep loss for short-term (24 and 96 h) and long-term (21 days) intervals, as well as their respective recovery periods in peripheral blood, brain, liver and heart tissue by the single cell gel (comet) assay. Rats were paradoxically deprived of sleep (PSD) by the platform technique for 24 or 96 h, or chronically sleep-restricted (SR) for 21 days. We also sought to verify the time course of their recovery after 24 h of rebound sleep. the results showed DNA damage in blood cells of rats submitted to PSD for 96 h. Brain tissue showed extensive genotoxic damage in PSD rats (both 24 and 96 h), though the effect was more pronounced in the 96 h group. Rats allowed to recover from the PSD-96 h and SR-21 days treatments showed DNA damage as compared to negative controls. Liver and heart did not display any genotoxicity activity. Corticosterone concentrations were increased after PSD (24 and 96 h) relative to control rats, whereas these levels were unaffected in the SR group. Collectively, these findings reveal that sleep loss was able to induce genetic damage in blood and brain cells, especially following acute exposure. Since DNA damage is an important step in events leading to genomic instability, this study represents a relevant contribution to the understanding of the potential health risks associated with sleep deprivation. (C) 2009 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Dept Psychobiol, BR-11060001 São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Dept Cardiovasc Physiol, BR-11060001 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Dept Psychobiol, BR-11060001 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Dept Cardiovasc Physiol, BR-11060001 São Paulo, SP, Brazil
dc.description.provenanceMade available in DSpace on 2016-01-24T13:52:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-04-30en
dc.description.sourceWeb of Science
dc.description.sponsorshipAssociacao Fundo de Incentivo a Psicofarmacologia (AFIP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCEPID
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 06/58274-5
dc.description.sponsorshipIDFAPESP: 2007/01228-4
dc.description.sponsorshipIDFAPESP: 07/566206
dc.description.sponsorshipIDCEPID: 98/14303-3
dc.format.extent562-567
dc.identifierhttps://dx.doi.org/10.1016/j.pnpbp.2009.02.014
dc.identifier.citationProgress in Neuro-psychopharmacology & Biological Psychiatry. Oxford: Pergamon-Elsevier B.V., v. 33, n. 3, p. 562-567, 2009.
dc.identifier.doi10.1016/j.pnpbp.2009.02.014
dc.identifier.issn0278-5846
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/31460
dc.identifier.wosWOS:000265470500025
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofProgress in Neuro-psychopharmacology & Biological Psychiatry
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectChronic sleep deprivationen
dc.subjectCorticosteroneen
dc.subjectDNA damageen
dc.subjectRatsen
dc.subjectRebounden
dc.subjectSingle cell gel (comet) assayen
dc.titleDistinct effects of acute and chronic sleep loss on DNA damage in ratsen
dc.typeResenha
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