A Treatment with a Protease Inhibitor Recombinant from the Cattle Tick (Rhipicephalus Boophilus microplus) Ameliorates Emphysema in Mice

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dc.contributor.authorLourenco, Juliana Dias
dc.contributor.authorNeves, Luana de Paiva
dc.contributor.authorOlivo, Clarice Rosa
dc.contributor.authorDuran, Adriana
dc.contributor.authorAlmeida, Francine Maria de
dc.contributor.authorArantes, Petra de Mello Motta
dc.contributor.authorPrado, Carla Máximo [UNIFESP]
dc.contributor.authorLeick, Edna Aparecida
dc.contributor.authorTanaka, Aparecida Sadae [UNIFESP]
dc.contributor.authorMartins, Milton de Arruda
dc.contributor.authorSasaki, Sergio Daishi
dc.contributor.authorLopes, Fernanda Degobbi Tenorio Quirino dos Santos
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal do ABC (UFABC)
dc.date.accessioned2016-01-24T14:37:24Z
dc.date.available2016-01-24T14:37:24Z
dc.date.issued2014-06-02
dc.description.abstractAims: To determine whether a serine protease inhibitor treatment can prevent or minimize emphysema in mice.Methods: C57BL/6 mice were subjected to porcine pancreatic elastase (PPE) nasal instillation to induce emphysema and were treated with a serine protease inhibitor (rBmTI-A) before (Protocol 1) and after (Protocol 2) emphysema development. in both protocols, we evaluated lung function to evaluate the airway resistance (Raw), tissue damping (Gtis) and tissue elastance (Htis). the inflammatory profile was analyzed in the bronchoalveolar lavage (BALF) and through the use of morphometry; we measured the mean linear intercept (Lm) (to verify alveolar enlargement), the volume proportion of collagen and elastic fibers, and the numbers of macrophages and metalloprotease 12 (MMP-12) positive cells in the parenchyma. We showed that at both time points, even after the emphysema was established, the rBmTI-A treatment was sufficient to reverse the loss of elastic recoil measured by Htis, the alveolar enlargement and the increase in the total number of cells in the BALF, with a primary decrease in the number of macrophages. Although, the treatment did not control the increase in macrophages in the lung parenchyma, it was sufficient to decrease the number of positive cells for MMP-12 and reduce the volume of collagen fibers, which was increased in PPE groups. These findings attest to the importance of MMP-12 in PPE-induced emphysema and suggest that this metalloprotease could be an effective therapeutic target.en
dc.description.affiliationUniv São Paulo, Dept Med, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biol Sci, São Paulo, Brazil
dc.description.affiliationUNIFESP EPM, Dept Bioquim, São Paulo, Brazil
dc.description.affiliationUFABC, Ctr Ciencias Nat & Humanas, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biol Sci, São Paulo, Brazil
dc.description.affiliationUnifespUNIFESP EPM, Dept Bioquim, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipLaboratorios de Investigacao Medica do Hospital das Clinicas da Faculdade de Medicina da USP (LIM/HC)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent9
dc.identifierhttps://dx.doi.org/10.1371/journal.pone.0098216
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 9, n. 6, 9 p., 2014.
dc.identifier.doi10.1371/journal.pone.0098216
dc.identifier.fileWOS000336956300048.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/37854
dc.identifier.wosWOS:000336956300048
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleA Treatment with a Protease Inhibitor Recombinant from the Cattle Tick (Rhipicephalus Boophilus microplus) Ameliorates Emphysema in Miceen
dc.typeinfo:eu-repo/semantics/article
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