A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice

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dc.citation.issue2
dc.citation.volume18
dc.contributor.authorTheodoro-Junior, Osmar Aparecido
dc.contributor.authorRighetti, Renato Fraga
dc.contributor.authorAlmeida-Reis, Rafael
dc.contributor.authorMartins-Oliveira, Bruno Tadeu
dc.contributor.authorOliva, Leandro Vilela
dc.contributor.authorPrado, Carla Maximo [UNIFESP]
dc.contributor.authorSaraiva-Romanholo, Beatriz Mangueira
dc.contributor.authorLeick, Edna Aparecida
dc.contributor.authorPinheiro, Nathalia Montouro
dc.contributor.authorLobo, Yara Aparecida [UNIFESP]
dc.contributor.authorMartins, Milton de Arruda
dc.contributor.authorVilela Oliva, Maria Luiza [UNIFESP]
dc.contributor.authorLopes Calvo Tiberio, Iolanda de Fatima
dc.coverageBasel
dc.date.accessioned2020-07-17T14:03:07Z
dc.date.available2020-07-17T14:03:07Z
dc.date.issued2017
dc.description.abstractProteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for emphysema. Our aim was to evaluate the effects of a plant Kunitz proteinase inhibitor, Enterolobium contortisiliquum trypsin inhibitor (EcTI), on several aspects of experimental elastase-induced pulmonary inflammation in mice. C57/Bl6 mice were intratracheally administered elastase (ELA) or saline (SAL) and were treated intraperitoneally with EcTI (ELA-EcTI, SAL-EcTI) on days 1, 14 and 21. On day 28, pulmonary mechanics, exhaled nitric oxide (ENO) and number leucocytes in the bronchoalveolar lavage fluid (BALF) were evaluated. Subsequently, lung immunohistochemical staining was submitted to morphometry. EcTI treatment reduced responses of the mechanical respiratory system, number of cells in the BALF, and reduced tumor necrosis factor- (TNF-), matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase-12 (MMP-12), tissue inhibitor of matrix metalloproteinase (TIMP-1), endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS)-positive cells and volume proportion of isoprostane, collagen and elastic fibers in the airways and alveolar walls compared with the ELA group. EcTI treatment reduced elastase induced pulmonary inflammation, remodeling, oxidative stress and mechanical alterations, suggesting that this inhibitor may be a potential therapeutic tool for chronic obstructive pulmonary disease (COPD) management.en
dc.description.affiliationUniv Sao Paulo, Sch Med, Dept Clin Med, BR-01246903 Sao Paulo, Brazil
dc.description.affiliationHosp Sirio Libanes, Phys Therapy Dept, BR-01308050 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biosci, BR-09972270 Diadema, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biochem, BR-09972270 Diadema, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biosci, BR-09972270 Diadema, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biochem, BR-09972270 Diadema, Brazil
dc.description.provenanceMade available in DSpace on 2020-07-17T14:03:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2017. Added 1 bitstream(s) on 2020-07-17T14:35:09Z : No. of bitstreams: 1 WOS000395457700171.pdf: 3577740 bytes, checksum: 25b83137ac47235b547eb6b488ba4c92 (MD5)en
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.3390/ijms18020403
dc.identifier.citationInternational Journal Of Molecular Sciences. Basel, v. 18, n. 2, p. -, 2017.
dc.identifier.doi10.3390/ijms18020403
dc.identifier.fileWOS000395457700171.pdf
dc.identifier.issn1422-0067
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55173
dc.identifier.wosWOS:000395457700171
dc.language.isoeng
dc.publisherMdpi Ag
dc.relation.ispartofInternational Journal Of Molecular Sciences
dc.rightsAcesso aberto
dc.subjectCOPDen
dc.subjectemphysemaen
dc.subjectproteinase inhibitoren
dc.subjectEcTIen
dc.titleA Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Miceen
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