An immunoproteomic approach revealing peptides from Sporothrix brasiliensis that induce a cellular immune response in subcutaneous sporotrichosis

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dc.citation.volumev. 8
dc.contributor.authorAlmeida, Jose Roberto Fogaca de
dc.contributor.authorJannuzzi, Grasielle Pereira
dc.contributor.authorKaihami, Gilberto Hideo
dc.contributor.authorBreda, Leandro Carvalho Dantas
dc.contributor.authorFerreira, Karen Spadari [UNIFESP]
dc.contributor.authorAlmeida, Sandro Rogerio de
dc.coverageLondon
dc.date.accessioned2020-07-20T16:31:16Z
dc.date.available2020-07-20T16:31:16Z
dc.date.issued2018
dc.description.abstractSporothrix brasiliensis is the most virulent fungus of the Sporothrix complex and is the main species recovered in the sporotrichosis zoonotic hyperendemic area in Rio de Janeiro. A vaccine against S. brasiliensis could improve the current sporotrichosis situation. Here, we show 3 peptides from S. brasiliensis immunogenic proteins that have a higher likelihood for engaging MHC-class II molecules. We investigated the efficiency of the peptides as vaccines for preventing subcutaneous sporotrichosis. In this study, we observed a decrease in lesion diameters in peptide-immunized mice, showing that the peptides could induce a protective immune response against subcutaneous sporotrichosis. ZR8 peptide is from the GP70 protein, the main antigen of the Sporothrix complex, and was the best potential vaccine candidate by increasing CD4(+) T cells and higher levels of IFN-gamma, IL-17A and IL-1 beta characterizing a strong cellular immune response. This immune environment induced a higher number of neutrophils in lesions that are associated with fungus clearance. These results indicated that the ZR8 peptide induces a protective immune response against subcutaneous sporotrichosis and is a vaccine candidate against S. brasiliensis infection.en
dc.description.affiliationUniv Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Dept Biol Sci, Diadema, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Dept Biol Sci, Diadema, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP
dc.description.sponsorshipIDFAPESP: 2016/04729-3
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1038/s41598-018-22709-8
dc.identifier.citationScientific Reports. London, v. 8, 2018.
dc.identifier.doi10.1038/s41598-018-22709-8
dc.identifier.fileWOS000426825900027.pdf
dc.identifier.issn2045-2322
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55829
dc.identifier.wosWOS:000426825900027
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofScientific Reports
dc.rightsAcesso aberto
dc.titleAn immunoproteomic approach revealing peptides from Sporothrix brasiliensis that induce a cellular immune response in subcutaneous sporotrichosisen
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